Pioneer Hi-bred International, Inc.

Emco Specialties, Inc.

Gary L Jaehnel

Jason M Cope

James L Hunter

Joshua Luke Mongan

Steven J Corak

Steven Michael Becker

Hugh Lu

James W Stevenson

John S Holloway

In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker.

Method and computer program product for distinguishing and sorting seeds containing a genetic element of interest

A system and method for preparing a sample of seeds or representative seed portions are provided. In various embodiments, the system and method include a force applying member and a seed container that includes at least one compartment containing a seed. The force applying member is configured to apply a force to the seed so as to break the seed into two or more seed particles, which in some embodiments may be collected in a seed particle collector. The present invention improves on the prior art by greatly reducing (and in some embodiments eliminating) the manual processes typically involved in generating tissue samples from seeds and preparing the tissue for genetic analysis. Additionally, the present invention is scaleable, and can be configured to generate samples from many seeds in a short period of time. The present invention also minimizes the risk of contamination and cross-contamination of the seed particles.

High-throughput, seed sampling and collection system and method

A method for distinguishing and sorting seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker. In some embodiments, the red fluorescent protein marker is discernable when excited by a certain energy and the evaluating step comprises exciting the seeds containing a genetic element of interest with the certain energy and detecting an emission resulting at least in part from the exciting step.

High throughput, seed sampling and collection system and method

A machine for placing weather stripping in an elongated C-shaped channel includes a press wheel which is rotatably mounted on a frame, and a plurality of guide wheels for guiding the channel in a longitudinal direction. The weather stripping is partially placed within the channel, and the press wheel forces the weather stripping completely into the channel as the channel is moved longitudinally pass the press wheel.

Growing community of inventors

Des Moines, IA, United States of America

Gary L Jaehnel