Virginia Commonwealth University

The Henry M. Jackson Foundation for the Advancement of Military Medicine Inc.

The University of Akron

Other

The University of Tennessee Research Foundation

University of Memphis Research Foundation

Organogenesis, Inc.

Gary L Bowlin

David Simpson

Gary E Wnek

Marcus E Carr, Jr

Kevin R Ward

Marcus E Carr

James Bowman

Robert F Diegelmann

Stanley E Rittgers

Stephen W Rothwell

Peter J Stevens

Jamil A Matthews

Saravanamoorthy Rajendran

Philippe Lam

Helen L Fillmore

Rao R Ivatury

Kelman I Cohen

James R Bowman

Raymond J Colello

Isaac Anthony Rodriguez

Brenton Walter Burger

Allison E Fetz

Wayne Barbee

Louis Terracio

Woon Chow

Stephen Rothwell

Marko Z Radic

Balendu Shekhar Jha

David G Simpson

The invention generally relates to dextran fibers which are preferably electrospun and devices formed from such fibers. In particular, such devices may include substances of interest (such as therapeutic substances) associated with the electrospun fibers. Upon exposure to a liquid the electrospun fibers dissolve immediately and the substances of interest are released into the liquid. Exemplary devices include bandages formed from electrospun dextran fibers and associated agents that promote hemostasis, such as thrombin and fibrinogen.

Electrospun dextran fibers and devices formed therefrom

As described below, the present invention features compositions and methods for inhibiting inflammation in connection with an acellular template (e.g., an electrospun template). In one embodiment, the template is impregnated with an agent (e.g., N-&#x3b1;-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide (Cl-amidine)) that inhibits a peptidylarginine deaminase (e.g., PAD4).

Compositions and methods for inhibiting inflammation

Compositions comprising clay minerals and methods for their use in promoting hemostasis are provided. The compositions comprise clay minerals such as bentonite, and facilitate blood clotting when applied to a hemorrhaging wound. Electrospun or electrosprayed materials (e.g. bandages, micron beads, etc.) which include clay minerals, and methods for the treatment of acute hemorrhage, are also provided.

Hemostasis of wound having high pressure blood flow

The invention features biodegradable materials, and in vitro and in vivo methods of using such compositions to promote bone and soft tissue growth and healing.

Compositions and methods for enhancing healing and regeneration of bone and soft tissue

Hemostasis of wound having high pressure blood flow using kaolin and bentonite

Nerve guides which are formed from three dimensional (3D) arrays of highly aligned electrospun fibers are provided. The electrospun fibers are oriented parallel to the long axis of the guide, and gaps and elongated spaces between the stacked fiber arrays provide channels for directed axonal growth. In some embodiments, the nerve guides also comprise high precision gradients of beneficial substances such as growth factors, which aid in nerve regeneration and growth along the guide.

Electrospun nerve guides for nerve regeneration designed to modulate nerve architecture

The invention is directed to formation and use of electroprocessed collagen, including use as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed collagen may also be combined with other molecules in order to deliver substances to the site of application or implantation of the electroprocessed collagen. The collagen or collagen/cell suspension is electrodeposited onto a substrate to form tissues and organs.

Electroprocessed collagen and tissue engineering

High-pressure bleeding wounds (and other bleeding wounds) may be treated by applying direct pressure directly in the bleeding wound, such as by applying a back pressure in a confined space around and in the wound. Certain substances and articles may be inserted into the wound, and the wound may be enclosed with that substance or article (such as a hemostatic substance, which may be polymeric), by swelling on contact with molecules (such as water molecules in the blood) encountered in the wound, generates the desired pressure to stop or at least reduce the bleeding without the detrimental effects of a tourniquet. Clot-inducing substances may be introduced into the wound contemporaneously with direct pressure application directly in the wound. Compressible and non-compressible wounds are treated. Treatment stops bleeding without producing pressure injury or ischemic damage. Medical devices using this technology are provided, including removable, biodegradable, medic-administrable devices.

Treatment for high pressure bleeding

The invention is directed to formation and use of electroprocessed fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed fibrin may also be combined with other molecules in order to deliver the molecules to the site of application or implantation of the electroprocessed fibrin. The fibrin or fibrin/cell suspension is electrodeposited onto a substrate to form the tissues and organs.

Electroprocessed fibrin-based matrices and tissues

The invention is directed to novel compositions comprising an electroprocessed material and a substance, their formation and use. The electroprocessed material can, for example, be one or more natural materials, one or more synthetic materials, or a combination thereof. The substance can be one or more therapeutic or cosmetic substances or other compounds, molecules, cells, vesicles. The compositions can be used in substance delivery, including drug delivery within an organism by, for example, releasing substances or containing cells that release substances. The compositions can be used for other purposes, such as prostheses or similar implants.

Electroprocessed material made by simultaneously electroprocessing a natural protein polymer and two synthetic polymers

The invention is directed to use of fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or &ldquo;organ-like&rdquo; tissue. A preferred embodiment is a plasma-derived fibrin matrix containing cells.

Plasma-derived fibrin-based matrices and tissue

A muscle implant includes an extracellular matrix, tendon and muscle cells. The extracellular matrix is made of a matrix of electrospun polymer fibers. The tendon is made of extruded collagen fibers and the muscle cells are disposed on the extracellular matrix in such a manner that the combination of components will functionally and structurally act as normal muscle tissue. Cardiac and smooth muscles may be similarly formed without tendons but including the extracellular matrix and muscle cells.

Engineered muscle

Growing community of inventors

Memphis, TN, United States of America

Gary L Bowlin