454 Life Sciences Corporation

Roche Molecular Systems, Inc.

Gary James Sarkis

John Harris Leamon

Jan Berka

Jonathan Marc Rothberg

Michael Weiner

Yi-Ju Chen

Kenton L Lohman

Vinod B Makhijani

Maithreyan Srinivasan

Steve Lefkowitz

Steven M Lefkowitz

Jonathan M Rothberg

Michael Egholm

Triantafyllos P Tafas

Petros Tsipouras

Michael Kilpatrick

Brian Christopher Godwin

Jan Fredrik Simons

Maithreyn Srinivasan

Zhoutao Chen

Stephen Kyle Hutchison

Automated methods for detecting cancer and related hyperplasias in biological samples.

Automated fluorescence microscopy method for determining efficacy of treatment of cancers and hyperplasias

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

Bead emulsion nucleic acid amplification

The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.

Paired end sequencing

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Guilford, CT, United States of America

Gary James Sarkis