Asuragen, Inc.

Applied Biosystems, LLC

Applera Corporation

Ambion, Inc.

Life Technologies Corporation

Gary J Latham

Liangjing Chen

Robert Setterquist

Sachin Sah

Jon Kemppainen

Brittan L Pasloske

Richard Conrad

Xingwang Fang

Richard Andrew Blidner

Matthew M Winkler

W Antoni Kudlicki

Ashish Choudhary

Andrew Hadd

Heidi J Peltier

Ru Cao

Jon A Kemppainen

Robert A Setterquist

Richard C Conrad

The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.

Methods of RNA amplification

Described herein are methods, compositions, kits, and uses thereof for analysis of nucleic acid segments comprising a repeating A/T-rich segment, wherein the repeating A/T-rich segment is: (i) a homopolymeric segment comprising at least 10 A residues, at least 10 T residues, or at least 10 U residues, wherein the at least 10 A, T, or U residues are consecutive or interrupted once by one to three other nucleotides; or (ii) a segment comprising (TA), (AT), (TA), or (AT), wherein n is 2 or greater and m is such that the length of the repeating A/T-rich segment is 10 or more residues.

Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating A/T-rich segments

This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.

PCR methods for characterizing the 5' untranslated region of the FMR1 and FMR2 genes

Methods are provided for determining the methylation status of GC-rich templates. The methods include use of GC reference standards that allow simultaneous characterization of methylation status and CGG repeat length. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome.

mPCR methods for analyzing repeat sequences

The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.

Modified surfaces as solid supports for nucleic acid purification

Disclosed herein are methods for the automated reconstruction of a genotype of a gene, fragment, or genomic region using exhaustive enumeration. The methods can be used to reconstruct the genotype of any GC-rich sequence, such as the CGG repeat region in the 5' UTR of FMR1 or the CCG repeat region in the 5&#x2032; UTR of FMR2. Also disclosed is an apparatus for use in conducting automated genotype reconstruction, as well as methods of diagnosis and treatment using exhaustive enumeration methods to reconstruct and identify genotypes associated with a disease or disorder.

Comprehensive FMR1 genotyping

The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

Recombinant reverse transcriptases

Methods are provided for increasing the processivity of DNA polymerases on GC-rich templates. The methods relate to providing enhancers and biased ratios of dNTPs, and may be used in DNA amplification reactions. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome.

Method of amplification of GC-rich DNA templates

Improved ball mill disruption techniques. In different embodiments, disrupting particles that are not substantially spherical are used. In other embodiments, roughened disrupting particles are used. In other embodiments, larger disrupting particles are used. In each instance, improved disruption can be achieved.

Biological sample disruption techniques

This application describes methods of quantifying a target miRNA in a biological sample by measuring the amounts of a target miRNA and at least one reference oncomir in a reaction volume, and normalizing the amount of target miRNA to the amount of one or more reference oncomirs.

Growing community of inventors

Austin, TX, United States of America

Gary J Latham