Ohio University

University of Nebraska

Gary D Meyer

Marcia J Kieliszewski

Jianfeng Xu

Patrick L Iversen

John J Kopchick

Thomas R Porter

Shigeru Okada

Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.

Nucleic acid for plant expression of a fusion protein comprising hydroxyproline O-glycosylation glycomodule

Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

Growth hormone and interferon-alpha 2 glycoproteins produced in plants

Targeted site specific antisense oligodeoxynucleotide delivery method

Growing community of inventors

Athens, OH, United States of America

Gary D Meyer