Berkeley Lights, Inc.

Bruker Cellular Analysis, Inc.

Gang F Wang

J Tanner Nevill

Andrew W McFarland

Mark P White

Randall D Lowe, Jr

Kevin T Chapman

Jason M McEwen

Aathavan Karunakaran

Russell A Newstrom

Darcy K Kelly-Greene

Xiao Guan

Daniele Malleo

Xiaohua Wang

Kristin G Beaumont

Natalie C Marks

Keith J Breinlinger

Minha Park

Peggy A Radel

Xiao Guan Radstrom

Guido K Stadler

George L Fox

Jian Gong

John A Tenney

I-Jong Lin

Troy A Lionberger

Gregory G Lavieu

Keith Joseph Breinlinger

Matthew E Fowler

Kevin D Loutherback

Jason M Mcewen

Phillip J M Elms

Anupam Singhal

Christine E Sun

Russell Newstrom

Andrew Mcfarland

Darcy Kelly-Greene

Jr Randall D Lowe

Andrew W Mcfarland

Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

Well plate incubator

Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Microfluidic cell culture

A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor

Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

Well-plate incubator

Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

Methods, systems and kits for in-pen assays

Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.

Microfluidic reporter cell assay methods and kits thereof

Growing community of inventors

Mountain View, CA, United States of America

Gang F Wang