Biosynth Ag

G&uuml;nter Schabert

Lawrence Restaino

Elon W Frampton

Detection of microbial metabolites

Potentially fluorogenic compounds and plating media containing same

The invention provides compounds of formula (I) in which R , R , R , R and R are independently selected from hydrogen and chromogenic substituents, and X is selected from the group consisting of hydroxyl; OR wherein R is selected from the group consisting of C -C alkyl; and O Me wherein Me is a cation derived from an organic or inorganic base. A preferred species of formula (I) is 4-Methylumbelliferyl myo-inositol-1-phosphate and salts thereof with an organic or inorganic base. The invention also provides fluorogenic methods for detecting various pathogenic bacteria, e.g., Listeria, Staphylococcus and Clostridium species, using substrates containing at least one formula (I) compound. A kit for detecting a phosphatidyl-inositol-specific phospholipase C enzyme as an indication of bacterial activity is disclosed.

Growing community of inventors

Goldach, Switzerland

Günter Schabert

Growing community of inventors

Goldach, Switzerland

G&uuml;nter Schabert

Günter Schabert