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Institut Fur Chemo-und Biosensorik Munster E.v.

G&ouml;ran Key

Andreas Katerkamp

Ulrich Kunz

Gabriele Chemnitius

Frank Grawe

The invention relates to a method for monitoring metabolic activity of cells in liquid media, to an appropriate vessel and to a device for implementing said method. The invention aimes at enhancing measuring accuracy and reducing the costs involved in monitoring oxygen consumption and thus metabolic activity in culture cells. To this end, oxygen concentration is optically determined with the aid of sensor membranes in a liquid medium located in partially oxygen permeable vessels. Then, the measured oxygen concentrations in the vessels containing culture cells in a liquid medium are compared with the measured oxygen concentration in a vessel containing only such a liquid medium without cells and/or with an oxygen concentration calculated using the measured values of other parameters.

Method, vessel and device for monitoring metabolic activity of cell cultures in liquid media

The invention concerns a device and a method for carrying out fluorescence immunoassays, wherein from at least one light source light is directed onto a surface at one end of a light waveguide and with the light coupled into the light waveguide by evanescent field excitation at the surface of the light waveguide fluorescence of at least one labelling substance bound to a chemical or biochemical partner of a general receptor-ligand system is excited. The solution according to the invention is here to provide a possible way of carrying out fluorescence immunoassays with high accuracy of measurement at low cost and within a short time. To achieve this object, the fluorescent light is decoupled from the light waveguide and directed via an optical system onto an optical detector with which the intensity of the fluorescent light is measured. Here the light waveguide is held in a measuring chamber formed in a piston of a piston and cylinder unit, and the measuring chamber is connected to the interior of the cylinder by an inlet formed in the piston.

Growing community of inventors

Osnabruck, Germany

Göran Key

Growing community of inventors

Osnabruck, Germany

G&ouml;ran Key

Göran Key