Biomerieux, Inc.

Gaël Kaneko

Pierre Mahe

Ghislaine Guigon

Mahendrasingh Ramjeet

Margaux Chapel

Maragux Chapel

A method for quantifying the sensibility of a test microorganism to a concentration of an antimicrobial agent includes: preparing two liquid samples including the microorganism, one having the antimicrobial agent and one without; for each sample acquiring, by a flow cytometer, a digital set values including a fluorescence, forward, or side scatter distribution, and computing: a first coordinate value corresponding to the acquired distribution main mode and an acquired distribution first area for values greater than the first coordinate value, and a second coordinate value, greater than the first, for which an acquired distribution second area between the values equals a first area predefined percentage over 50%; computing a ratio according to: where Mode(ATB) and QT(ATB) are the first and second coordinate values with the antimicrobial agent concentration, and Mode(no ATB) and QT(no ATB) are respectively the first and second coordinate values without the antimicrobial agent concentration.

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Marcy l'Etoile, France

Gaël Kaneko