Centre National De La Recherche Scientifique

Institut National De La Recherche Agronomique

Institut National Des Sciences Appliquees De Toulouse

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Gabrielle Potocki-Veronese

Magali Remaud-Simeon

Claire Moulis

Pierre Monsan

Pierre F Monsan

The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the &#x3b1;-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of &#x3b1;-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.

Construction of new variants of dextransucrase DSR-S by genetic engineering

Growing community of inventors

Lautignac, France

Gabrielle Potocki-Veronese