The University of New Mexico

Duke University

Other

Harvard College

Massachusetts Institute of Technology

Children's Medical Center Corporation

Intel Corporation

Sandia Corporation

President and Fellows of Havard College

Wang, Qiming

Lopez, Gabriel, P.

Levering, Vrad, W.

Shivapooja, Phanindhar

Zhao, Xuanhe

Gabriel P Lopez

Linnea K Ista

Steven Roy Julien Brueck

George M Whitesides

Donald Elliot Ingber

Ashutosh Chilkoti

Amit Kumar

Gregory N Stephanopoulos

Rahul Singhvi

Plamen B Atanassov

Menake E Piyasena

Daniel I C Wang

Venkata Goparaju

David G Whitten

Kirk S Schanze

Hongyou Fan

Dimiter N Petsev

Motokatsu Ogawa

Steven Wayde Graves

Michael J O'Brien

Reema Zeineldin

Mangesh Bore

Yunfeng Lu

Charles Jeffrey Brinker

Thomas S Corbitt

Daniel I Wang

Nick Carroll

Ali Ghoorchian

Joseph R Simon

Robert W Applegate, Jr

Aurelio Evangelista Lara

Sireesha Chemburu

Gautam Gupta

Emmanuel Rabinovich

Charles Wyatt Shields, Iv

Gregory Kaduchak

C Jeffrey Brinker

Larry A Sklar

Stephen D Hersee

Sang M Han

Ravinder K Jain

Leah Marie Johnson

Cornelius F Ivory

Abhaya K Datye

Eunkyung Ji

Scott A Sibbett

Xuanhe Zhao

Andrew P Shreve

Timothy L Ward

Felipe Garcia Quiroz

Kateryna Artyushkova

Zhijun Zhou

Yanli Tang

David Keller

Shailendra Rathod

Hien Pham

Nick Kirby

Vrad W Levering

Phanindhar Shivapooja

Qiming Wang

Lu Gao

Pearlson Prashanth Austin Suthanthiraraj

Brett Andrzejewski

Kevin Cushing

Ramanathan Nagarajan

Plamen Atanasov

Jaime Bravo

Anthony L Garcia

Paul Bisong

Dmitri A Brevnov

Marcos Barela

Nick J Carroll

Philip Hampton

Leonard Tender

Kimberly Opperman

Juchao Yan

Frank Anthony Fencl

Jacqueline De Lora

Methods and apparatus for acoustically assisted droplet generation with or without cell encapsulation.

Frequency controlled mechanical actuator with microfluidic flow for generation of 3D microgel for cell culturing

Nano- to microscale liquid coacervate particles are provided. The liquid coacervate particles are produced by a process including stimulating a population of liquid droplets containing one or a mixture of components to induce a phase separation point of a first component, and maintaining stimulation at the phase separation point to form a coacervate domain of the first component within each of the droplets to form the liquid coacervate particles. The self-assembled nano, meso, micro and macro liquid coacervate particles and related coated substrates can have utility in drug delivery, bioanalytical systems, controlled cell culture, tissue engineering, biomanufacturing and drug discovery.

Programmable liquid, gel and biohybrid compartments and methods of use

The presently disclosed subject matter relates to modular peptide-substrate protease assays, in particular low cost and reliable methodology for measurement of protease and other enzyme activity that may be detected by optical turbidimetry or visual observation. The presently disclosed subject matter also relates to methods of using the disclosed assays within methods of detecting and monitoring diseases, methods of drug discovery, as well as in detection devices and systems.

Systems and devices for protease detection based on engineered polymers and biopolymers and methods of use

A kit for detection of an analyte of interest in a liquid sample, and methods of using, are provided. The kit may include a pipette and a disposable pipette tip configured to engage the pipette. The pipette tip may define an acoustic channel configured for allowing flow-through of a liquid. The kit may also include a vibratory device in communication with the acoustic channel and configured for imparting a vibratory force thereto. The impartation of the vibratory force may create standing acoustic waves, thereby separating any negative acoustic contrast particles (NACPs) from the remaining contents of the liquid sample. The NACPs may capable of biospecific recognition of the analyte of interest, thereby separating the analytes of interest, which can then be collected or analyzed accordingly.

Disposable pipette tip and methods of use

A composition and method is provided for synthesizing silicone particles with narrow size distributions and tunable degrees of crosslinking for encapsulation of an active ingredient or multiple active ingredients in the matrix of the particles for controlled release. The method comprises agitating at least one type of silane monomer having two, three or four siloxane bonds in an acidic aqueous solution under conditions sufficient to allow for hydrolysis of the monomers, adding a catalyst and a solution comprising a co-solvent and the active ingredient(s) to the acidic aqueous solution, and continuing to agitate the combined solution to allow for a condensation reaction and formation of silicone particles with a narrow size distribution encapsulating at least a portion of the active ingredient(s). The tunable degree of crosslink density of the particles formed is based on the ratios of the monomers used, which enables controlled release of the active ingredient(s) from the particles.

Compositions, systems and methods for the encapsulation and delivery of a substance

Disclosed herein are devices and methods for active biofouling control. According to an aspect, a device comprising a surface for contacting a biological material. The device also comprises a mechanism comprising a structure configured to change the surface between a first shape and a second shape. The change from the first shape to the second shape deforms the surface beyond a critical strain for debonding of a fouling agent from the surface when the fouling agent has bonded to the surface in the first shape.

Systems and methods for active biofouling control

The presently disclosed subject matter is directed to compositions, methods, and systems (e.g., platforms) comprising the same, the systems comprising, consisting of, or consisting essentially of micro- and macro-hydrogels that are formed from polypeptide micelles. The systems have enhanced mechanical properties that can be useful in a wide variety of applications, can be used for controlled release of drug-loaded micelles, and can be designed to reversibly assemble and disassemble on demand.

Hydrogels formed from polypeptide micelles and methods of use thereof

Acoustically responsive particles and methods are provided for their use. Methods are provided for making and using tunable, monodisperse acoustically responsive particles and negative contrast acoustic particles, wherein the particles can contain a functional group available for covalent modification.

Acoustically responsive particles

An affordable flow cytometry system with a significantly increased analytical rate, volumetric sample delivery and usable particle size including a light beam that interrogates multiple flow streams so as to provide excitation across all of the streams, and an optical objective configured to collect light from the sample streams and image the light onto an array detector.

Spatially correlated light collection from multiple sample streams excited with a line focused light source

We describe methods for synthesis and formulations of stable elastomeric negative acoustic contrast particles with controllable compressibility and density. These elastomeric negative acoustic contrast particles have a density/compressibility ratio that is less than that of water and therefore exhibit negative acoustic contrast under acoustic radiation exposure. This negative acoustic contrast allows our elastomeric negative acoustic contrast particles to be acoustically manipulated (e.g. separated) differently from other components (e.g. cells) within an aqueous solution. This disclosure also describes methods for biofunctionalization of the elastomeric negative acoustic contrast particles and as an example their use as platforms for bioassays. Potential applications of these elastomeric negative acoustic contrast particles include sensitive bioassays based on acoustic flow cytometry and other types of techniques that utilize acoustic fields, including ultrasound imaging and ultrasound triggered drug delivery.

Stable elastomeric negative acoustic contrast particles and their use in acoustic radiation fields

An analytical device such as a flow cytometer is provided in which a fluid sample flowing through a channel is focused into multiple, parallel particle streams by an acoustic wave field extending across the channel. Each stream is then presented to an individual detector to allow for simultaneous interrogation of the multiple streams and thus, high-throughput analysis of the fluid sample.

Multinode acoustic focusing for parallel flow cytometry analysis applications

Device and method for detecting the presence of known or unknown toxic agents in a fluid sample. Targets in the sample are bound to releasable receptors immobilized in a reaction region of a micro- or nano-fluidic device. The receptors are selected based on their affinity for classes of known toxic agents. The receptors are freed and the bound and unbound receptors separated based on differential electrokinetic mobilities while they travel to a detection device.

Integrated affinity microcolumns and affinity capillary electrophoresis

Novel compounds generally referred to herein as cationic oligomeric phenylene ethynylenes (OPEs), methods of synthesizing OPEs and various uses for the OPEs are described. The compounds can be synthesized in both symmetrical (S-OPE) and non-symmetrical (N-OPE) forms. Suitable uses include sensor and biocidal applications. Reusable structures incorporating the OPEs that are able to capture and release biological species of interest are also described.

Structure, synthesis, and applications for oligo phenylene ethynylenes

Growing community of inventors

Durham, NC, United States of America

Gabriel P Lopez