Chinoin Gyogyszer Es Vegyeszeti Termekek Gyara Rt.

Reanal Finomvegyszergyar

Gabor Seres

Jozsef Szejtli

Sandor Piukovich

Laszlo Boross

Miklos Jarai

Ivan Daroczi

Maria Szigetvari Nee Gabanyi

Bela Szanjani

Katalin Ivony Nee Kaldive

The invention relates to a process for the preparation of high-purity gamma- and alpha-cyclodextrin starting from a starch substrate of low reducing capacity, partially hydrolysed with alpha-amylase. According to the invention a starch substrate of low reducing capacity partially hydrolysed with alpha-amylase is converted with cyclodextrin glucosyl transferase, after the addition of an aliphatic ketone of the formula R.sub.1 COR.sub.2, in which R.sub.1 and R.sub.2 stand for a straight or branched chained alkyl group having from 1 to 6 carbon atoms, and/or a phenol derivative of the formula R.sub.3 R.sub.4 PheOH, wherein R.sub.3 and R.sub.4 are hydrogen, alkyl having from 1 to 4 carbon atoms, or R.sub.3 and R.sub.4 together form phenyl group, Phe stand for phenyl group, OH is hydroxyl and/or a benzene derivative of the formula R.sub.5 R.sub.6 Phe, wherein R.sub.5 and R.sub.6 are hydrogen, alkyl having from 1 to 4 carbon atoms, or R.sub.5 and R.sub.6 together form phenyl group, Phe is phenyl, as a precipitating agent, and if desired, alpha- and/or gamma-cyclodextrin is isolated from the conversion mixture in a crude form, or after further purification, in a pure form.

Process for the preparation of high-purity gamma- and alpha-cyclodextrins

The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme. The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained. The advantage of the process of the present invention is that the thus immobilized enzyme--contrary to the dissolved enzyme--is suitable for longlasting application and can be readily used on industrial scale continuous production.

Process for the immobilization of cyclodextrine glycosyltransferase

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Gabor Seres