Agrivida, Inc.

Ciba-geigy Corporation

Gabor Lazar

R Michael Raab

Binzhang Shen

Humberto De La Vega

James Reasoner Apgar

Philip A Lessard

Jason Eric Donald

Ingo Potrykus

Thomas Hohn

Jerzy Paszkowski

Hideaki Shinshi

Isabelle Rauseo

Michael R Raab

Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.

Animal feed with engineered phytases

Engineered phytases in animal feed

Methods for producing a trans-splicing intein-modified protease with enhanced solubility and regulating its activity are described. Intein-modified proteases having enhanced solubility and polynucleotides encoding the same are provided. Methods of storing trans-splicing proteases are also described.

Methods and compositions for stabilizing trans-splicing intein modified proteases

Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described.

Intein-modified proteases, their production and industrial applications

Engineered phytases and methods of using the same

Intein modified proteins, isolated nucleic acids encoding intein modified proteins, fragments of intein modified proteins, isolated nucleic acids encoding fragments of intein modified proteins, transgenic plants containing any of the foregoing, and antibodies recognizing epitopes on intein modified proteins are provided.

Intein-modified enzymes, their production and industrial applications

Methods for regulating protein activity by fusing dimerization domains to target protein are provided. Chimeric proteins that include dimerization domains fused to target proteins for altering the activity of the target proteins are described. Engineered nucleic acids encoding chimeric proteins and hosts engineered to express engineered nucleic acids are also provided.

Use of dimerization domains for temperature regulation of enzyme activity

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-&#x3b2;-sheet junction or a loop-&#x3b1;-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

Intein-modified enzymes, their production and industrial application

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-&#x3b2;-sheet junction or a loop-&#x3b1;-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

A process for the maintenance and proliferation of defective, non-infectious viruses or virus genomes, comprising mutations, i.e. deletions, substitutions, insertions and new rearrangements of the viral genes or combinations of the virus DNA with heterologous genetic material in proliferating plant material and for the regeneration of whole plants containing stably integrated into their genome said defective, non-infectious virus DNA.

Growing community of inventors

Belmont, MA, United States of America

Gabor Lazar