Ciba-geigy Corporation

Agri-diagnostics Associates

G David Grothaus

Frank P Petersen

Sally A Miller

James H Rittenburg

Mark D Clymer

Dennis R Stocker

Richard K Lankow

Stephanie L Papa

James Donovan

Douglas Malik

The invention relates to monoclonal antibodies that react specifically with members of the genus Mycosphaerella and hybridomas that produce such antibodies. The invention is further directed to a method for making a hybridoma cell line that produces monoclonal antibodies that react specifically with at least one species of Mycosphaerella, and a method of obtaining monoclonal antibodies therefrom. Methods and kits for diagnosing Mycosphaerella infections in plant material using such monoclonal antibodies are also within the scope of the invention.

Monoclonal antibodies to mycosphaerella species

A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.

Method and a kit for diagnosing plant diseases

The invention relates to monoclonal antibodies that react specifically with members of the genus Leptosphaeria and hybridomas that produce such antibodies. The invention is further directed to a method for making a hybridoma cell line that produces monoclonal antibodies that react specifically with Leptosphaeria nodorum, and a method of obtaining monoclonal antibodies therefrom. Methods and kits for diagnosing Leptosphaeria infections in plant material using such monoclonal antibodies are also within the scope of the invention.

Monoclonal antibodies to Leptosphaeria nodorum

Kit for diagnosing plant diseases

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Delran, NJ, United States of America

G David Grothaus