Asahi Glass Company, Limited

Jmtc Enzyme Corporation

Agc Inc.

Futoshi Hara

Shuichiro Kimura

Takayuki Tanaka

Hideki Tohda

Yuko Hama

Tetsuya Kotani

Mayumi Kobayashi

Susumu Zen-In

Ayako Kashima

Yuichiro Hagiya

The present invention relates to a transformant which usesas a host into which a D-LDH gene derived from bacteria of the genusand a D-LDH gene derived from bacteria of the genusare incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of thehost have been deleted or inactivated.

Transformant and process for production thereof, and process for production of lactic acid

The object is to provide an expression vector capable of expressing with a higher expression efficiency a protein derived from a foreign structural gene by genetic engineering using a yeast of the genusas a host, a cloning vector to produce the expression vector, a method for producing the expression vector, a transformant containing an expression cassette of the expression vector, a method for producing the transformant, and a method for producing a protein using the transformant. A cloning vector comprising a promoter capable of functioning in a yeast of the genus, a cloning site for introducing a foreign structural gene which is located downstream from the promoter and is regulated by the promoter, and ihc2 gene terminator of the yeast of the genus, and an expression vector comprising the promoter, the foreign structural gene and the ihc2 gene terminator.

Cloning vector

Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant comprising 3 copies of a human lactate dehydrogenase gene that is introduced into ahost, wherein a gene encoding pyruvate decarboxylase 2 of thehost is deleted or inactivated.

Transformant and its production process, and method for producing lactic acid

Provided is a transformant ofmutant which can produce and collect &#x3b2;-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus. The transformant of amutant of the present invention is a transformant of amutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a &#x3b2;-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus, and is useful for transforming a yeast of the genus, an expression vector, a transformant, etc.

[object Object]

Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in whichis used as a host, a lactate dehydrogenase gene ofis introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in thehost is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.

Transformant and method for producing same, and method for producing lactic acid

The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced intoas a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in thehost is deleted or inactivated.

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Tokyo, Japan

Futoshi Hara