Takara Shuzo Co., Inc.

Fujita Health University

Fusao Kimizuka

Ikunoshin Kato

Yoh'ichi Ohdate

Shouichi Goto

Tatsuru Kinoshita

Yuki Taguchi

Kimikazu Hashino

Yasutoshi Kawase

Kiyozo Asada

Takashi Uemori

Nobuto Koyama

Ichiro Azuma

Yoshizumi Ishino

Yoshikazu Kurosawa

Hirokazu Kotani

Yoshiko Nomura

Masamitsu Shimada

Masahiro Endo

Ikuo Saiki

Kiyotoshi Sekiguchi

Yoshihito Yaoi

Mika Hatai

Yuki Sugahara

Hidetaka Hashi

Koiti Titani

A new restriction endonuclease capable of recognizing the nucleotide sequence of the following formula 1 in double stranded DNA. ##STR1## and specifically cleaving it is disclosed. The endonuclease may be made cultivating a strain of the genus Streptomyces (FERM BP-4836) capable of producing it.

Restriction endonuclease

This invention provides an antibody having antigen binding activity and which has a heavy chain constant region into which has been introduced an RGDS amino acid sequence giving the antibody affinity for cells, including macrophages. The antibody exhibits artificial cell adhesive activity which is the newly expressed activity that results from insertion of the RGDS amino acid sequence. This antibody having artificial cell adhesive activity can accelerate the phagocytosis of macrophages, and can activate other effector cells. Therefore, this antibody contributes to the self-defense mechanism.

Artificial antibody

There is provided a polypeptide having the following formula (I'), and which polypeptide is referred to as Glu-OC and a process for the preparation thereof. ##STR1##

Polypeptides and method for preparing the same

This present invention is directed to an artificial functional polypeptide represented by the following structural formula [I]:

Polypeptide having human fibronectin-like cell adhesive activity

This invention relates to a functional polypeptide containing the binding domain of human fibronectin and the heparin-binding domain of human fibronectin.

Functional polypeptide containing a cell binding domain and a heparin

Polypeptides are provided which have a calpain inhibitory activity. The polypeptides have an activity like that of natural human calpastatin but are significantly shorter in length than natural human calpastatin. The polypeptides are suited for synthetic manufacture.

Human calpastatin-like polypeptide

The class II restriction endonuclease Sse 8387I is obtained from Streptomyces Sp 837 (FERM BP-3028). It recognizes the nucleotide sequence 5'-CCTGCA.dwnarw.GG-3' and cleaves at the position indicated by the arrow.

Restriction enzyme Sse 8387I

A polypeptide having the cell-spreading activity of human fibronectin. Methods of preparing the polypeptide are described.

Polypeptide with cell-spreading activity

Polypeptides with cell-spreading activity

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Ohmihachiman, Japan

Fusao Kimizuka