Sigma-aldrich Co. LLC

Sangamo Therapeutics, Inc.

Fuqiang Chen

Gregory D Davis

Timothy Seebeck

Xiao Ding

Yongmei Feng

Carol A Kreader

Brian W Ward

Thomas Wechsler

Kevin Bernard Ray

Qiaohua Kang

Shondra M Pruett-Miller

David Eric Cutter

Carol Kreader

Scott W Knight

Engineered Cas9 systems that utilize alternate protospacer adjacent motifs for target DNA binding, nucleic acids encoding the engineered Cas9 systems, and methods of using the engineered Cas9 systems for modifying target chromosomal sequences in eukaryotic cells.

Engineered CAS9 systems for eukaryotic genome modification

Compositions and methods for using programmable DNA binding proteins to increase the efficiency and/or specificity of targeted genome modification or to facilitate the detection of specific genomic loci in eukaryotic cells.

Using programmable DNA binding proteins to enhance targeted genome modification

Compositions and methods for using nucleosome interacting protein domains to increase accessibility of programmable DNA modification proteins to target chromosomal sequences, thereby increasing efficiency of targeted genome/epigenetic modification in eukaryotic cells.

Using nucleosome interacting protein domains to enhance targeted genome modification

Engineered Cas9 systems are disclosed herein.

CRISPR/Cas fusion proteins and systems

Engineered Cas9 systems are disclosed herein. For example, Cas9-marker fusion proteins are provided. Peptide linkers which facilitate fusion of heterologous proteins to CRISPR proteins in ways that preserve CRISPR functionality are also provided.

The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.

CRISPR-based genome modification and regulation

Processes and kits for preparing a plurality of amplification products with reduced non-specific amplification artifacts.

Nucleic acid amplification and library preparation

Methods and compositions for increasing nuclease-mediated genomic modification using DNA repair inhibitors are provided.

Methods and compositions for enhancing nuclease-mediated gene disruption

The present invention provides methods and kits for editing specific chromosomal sequences in cells. In particular, targeting endonucleases and single-stranded nucleic acids are used to edit the chromosomal sequence.

Growing community of inventors

St. Louis, MO, United States of America

Fuqiang Chen