Terumo Kabushiki Kaisha

Dai Nippon Printing Co., Ltd.

Osaka University

Nagasaki University

Fumiya Ohashi

Yoshiki Sawa

Ryohei Takeuchi

Shigeru Miyagawa

Masatoshi Kuroda

Kouichirou Yori

Kenji Oyama

Yoshitaka Maeda

Ryoma Bise

Kazumasa Yamaki

Hiroko Iseoka

Susumu Eguchi

Masaki Matsumura

Shun Yamaguchi

A method for producing a cell culture containing desired cells is provided. The desired cells are induced to be differentiated from pluripotent stem cells. The method includes seeding a cell population by dispersing embryoid bodies obtained by inducing differentiation from the pluripotent stem cells to the desired cells. The embryoid bodies are dispersed at a density reaching confluence or higher.

Method for increasing the proportion of desired cells from induced pluripotent stem cells

[Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation systemdetects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.

Image analysis system, culture management system, image analysis method, culture management method, cell group structure method, and program

Growing community of inventors

Kanagawa, Japan

Fumiya Ohashi