Konica Minolta, Inc.

Fuminori Okada

Masaru Takahashi

Noriaki Ohuchi

Kohsuke Gonda

Kensaku Takanashi

Mika Watanabe

Hideki Gouda

Takuji Aimiya

A method of storing fluorescent dye-containing resin particles comprising adding the fluorescent dye-containing resin particles in a liquid comprising a buffer, a protein, and a surfactant, thereby obtaining a particle-containing liquid. The rate of change in the backscatter intensity (transmitted light) at the center of the height of the particle-containing liquid left to stand for 24 hours after the adding is not less than &#x2212;1% based on the particle-containing liquid immediately after the adding.

Medium for resin particles containing fluorescent dye

For fluorescent nanoparticles having a zeta potential of &#x2212;10 mV to &#x2212;60 mV at pH 7.0 or a zeta potential of 0 mV to &#x2212;10 mV in a buffer of pH 6.0 to 8.0, an appropriate electrical repulsive force can be generated between biomolecules that are generally negatively charged and the fluorescent nanoparticles. As a result, non-specific binding between the fluorescent nanoparticles and the biomolecules is suppressed and the fluorescent nanoparticles are specifically bound to a biomolecule to be stained through interaction stronger than the electrical repulsive force, so that the visibility of the specific biomolecule to be stained can be improved. Further, since an appropriate electrical repulsive force is also generated between the fluorescent nanoparticles themselves, aggregation of the fluorescent nanoparticles can be inhibited and the dispersibility in a staining solution can thereby be maintained.

Fluorescent nanoparticles for biomolecular staining and manufacturing method for same

An object of the present invention is to provide a method of detecting a specific tissue or cell in a sample tissue section and accurately specifying both the position(s) and amount of a biological substance of interest that is expressed on the specific tissue or cell. The method of quantifying a biological substance in a tissue section according to the present invention comprises: (1) performing bright-field observable immunostaining that specifically stains a first biological substance in the tissue section (first immunostaining); (2) performing immunostaining with a fluorescent substance-containing nanoparticle that specifically stains a second biological substance in the tissue section (second immunostaining); (3) specifying the expression position(s) of the second biological substance in the tissue section by comparing the position of a stained image of the first immunostaining and the position of a stained image of the second immunostaining; and (4) determining the expression amount of the second biological substance by measuring the fluorescence intensity of the stained image of the second immunostaining.

Method for determining quantity of biological material in tissue section

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Tokyo, Japan

Fuminori Okada