Panasonic Corporation

Matsushita Electric Industrial Co., Ltd.

Phc Holdings Corporation

Panasonic Healthcare Holdings Co., Ltd.

Panasonic Healthcare Co., Ltd.

Fumihisa Kitawaki

Hirotaka Tanaka

Masataka Nadaoka

Mie Takahashi

Hiroshi Nakayama

Tatsurou Kawamura

Akihito Kamei

Masanori Tanaka

Kenji Watanabe

Jinsei Miyazaki

Keiko Yugawa

Nobuyuki Shigetoh

Masahiro Kouge

Nobuyuki Shigetou

Kazuyoshi Mori

Keigo Haneda

Kazuaki Edagawa

Seiji Onishi

Takako Matsumura

Chizu Asahara

The present invention forms a detection layer in an embedded biosensor probe by using a phenazine derivative as a redox mediator in which a phenazine group is covalently bonded to a high molecular weight polymer having a carboxyl group or an amino group, such as polyamino acid, polyimine, or polyallylamine; and the distance between the phenazine group and the high molecular weight polymer main chain is increased by using a polyethylene glycol chain.

Sensor using phenazine derivative or high molecular weight redox polymer containing phenazine derivative

A biosensor chip is placed in a biosensor device and is rotated while a biochemical analysis specimen is measured, the biosensor chip comprising a main body, a holding chamber, a dispensing chamber, a plurality of quantification chambers, and a plurality of measurement chambers. The main body has an inlet into which the specimen is poured. The holding chamber holds the poured specimen inside the main body. The dispensing chamber is connected to the holding chamber via a first channel and dispenses the specimen. The quantification chambers are connected to the dispensing chamber, hold a specific amount of the dispensed specimen, and are disposed at positions located away from a rotational center of a rotary motion according to a distance from the first channel. The measurement chambers are connected to the quantification chambers via a second channel and react the specimen with a biochemical analysis reagent.

Biosensor chip, and biosensor device equipped with same

Hemoglobin in a sample solution is quickly and reliably denatured; at the same time, quick and accurate measurement of hemoglobin and a hemoglobin derivative is realized. In a method for measuring hemoglobin and a hemoglobin derivative, and a reagent composition, a measurement kit, an analysis device, and an analysis system used in the method, a sample solution containing a blood component is treated with a nonionic surfactant, an oxidizing agent, and a metal salt to denature hemoglobin in the sample solution to measure the hemoglobin, and thereafter the amount of a hemoglobin derivative in the sample is measured by an immunological method using an antibody specifically binding to a denatured site of the denatured hemoglobin derivative.

Method for producing agglutinating reagent, agglutinating reagent or product produced thereby, and method for measuring analysis object using the same, and test kit and analysis device

A sample solution including blood components is processed with a denaturalization reagent comprising a nonionic surface-activating agent and an oxidizing agent to denaturalize a hemoglobin derivative in the sample solution, and thereafter, an immunoassay is performing utilizing an antibody that is specific to a denaturalized site of the hemoglobin derivative to measure the amount of the hemoglobin derivative in the sample. Therefore, when performing assay of hemoglobin derivative, denaturalization of hemoglobin can be performed speedily and reliably while minimizing adverse effects of the denaturalization reagent on immune reaction.

Hemoglobin derivative measurement method, and reagent composition, measurement kit, analysis device and analysis system for use in the method

According to the biosensor and the blood component analytical method of the present invention, in a biosensor that is made of a single layer or plural layers of a porous material as shown in FIG., having a reagent holding part and utilizing chromatography, a cell shrinkage reagent is carried on at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part. According to the biosensor having the above-mentioned structure and the blood component analytical method, even when whole blood is a sample, a high-accuracy blood component analysis cart be performed easily and quickly with less cost.

Biosensor and blood component analytical method

In a biosensor having a developing layer for developing a sample solution, including a reagent part immobilized to a portion of the developing layer and a marked reagent part which is held in a dry state by a portion of the developing layer, and is dissolvable by developing the sample solution, and qualitatively or quantitatively analyzing an analyte in the sample solution by measuring the amount of the marker reagent bound to the reagent immobilization part; wherein plural reagent immobilization parts exist, and the respective reagent immobilization parts have different affinities for the analyte in the sample solution or the marker reagent, whereby a prozone phenomenon can be detected. Further, a biosensor with a high precision of measurement, which has a wider dynamic range for the concentration of the analyte in the sample solution, can be provided. Thereby, a highly accurate and precise biosensor which can detect a prozone phenomenon even when the range of the concentration of the analyte in the sample solution is wide, and has a wide measurement dynamic range can be provided.

Biosensor and measurement method

A method capable of stirring a liquid sample and a reagent promptly and easily includes the following steps (A) and (B). In step (A), using a cell including: a liquid sample retaining section having a plurality of particles; and a liquid sample supply inlet, a liquid sample containing an analyte is supplied from the liquid sample supply inlet to the liquid sample retaining section. In step (B), the liquid sample and a specific binding substance capable of specifically binding to the analyte are stirred by the movement of the particles caused by the flow of the liquid sample in the cell resulting from the supply of the liquid sample, to obtain a liquid mixture. The electric charge of at least the surface of the particles and the electric charge of the specific binding substance have the same polarity in the liquid mixture.

Stirring method, cell, and measuring apparatus using the same

Herein disclosed is a liquid homogenizer for mixing two or more liquids, comprising: a rotator () rotatable around a rotation axis; at least two liquid-mixing chambers () formed in the rotator, and being different from each other in distance from the rotation axis; and at least two channels () through which one of the liquid-mixing chambers is communicated with the other of the liquid-mixing chambers (), wherein the liquid-mixing chambers () include an outer liquid-mixing chamber () and an inner liquid-mixing chamber () close to the rotation axis in comparison with the outer liquid-mixing chamber, when the rotator () is rotating around the rotation axis, the liquids are shifted by a centrifugal force to the outer liquid-mixing chamber from the inner liquid-mixing chamber through the channels (), and agitated to be mixed by turbulent flows in the outer liquid-mixing chamber.

Liquid homogenizer and analyzer employing the same

A sensor capable of measuring a plurality of measuring items quickly and accurately, a measuring device, and a measuring method are provided. The sensor includes a sample-holding unit for holding a sample containing an analyte; a sample-supplying port for supplying the sample to the sample-holding unit; a detecting unit for carrying out an electrochemical measurement, the unit being provided in the sample-holding unit; an optical measuring unit for carrying out an optical measurement, the unit being provided in the sample-holding unit; and a reagent-holding unit for holding a reagent for the optical measurement, the unit being provided in the sample-holding unit; wherein in the flowing direction of the sample supplied from the sample-supplying port in sample-holding unit, the sample-supplying port, the detecting unit, and the reagent-holding unit are positioned in the order recited.

Sensor, measuring device, and measuring method

A biosensor is provided with an area in which a reagent having bleaching action is carried in a state where it can be dissolved, at least in a part of a sample application area to which an inspection target solution is applied or the downstream of the sample application area in the direction of the inspection target solution permeating, in a development layer. In the so-constituted biosensor, a colored component in the inspection target solution can be faded by the bleaching reagent, so that the color in parts other than a reactive area is reduced in consequence, whereby a visual judgment is possible and a more accurate measurement result in which a reading error by a measuring device is extremely suppressed can be obtained.

Biosensor

A biosensor is provided with a development layer for developing an inspection target solution, that has a reagent immobilization part where an antibody for a measurement target in the inspection target solution is immobilized and a reagent holding part which marks in a dry state and holds an antibody which can be eluted by development of the inspection target solution in parts of the development layer. The biosensor measures a bonding amount of the marker reagent bonded to the reagent immobilization part, thereby qualitatively or quantitatively measuring components to be measured in the inspection target solution, and is further provided with a space forming material that forms a cavity part, which is a space into which the inspection target solution flows, between the development layer for developing the inspection target solution and the space forming material.

It is intended to provide an antibody specific to HbA1c, antibody-producing cells capable of supplying the antibody in a stable state in the future, and a method of constructing the antibody-producing cells without any probability factors, and a method which comprises fusing mouse spleen cells, which have been sensitized with an immunogen composed of a compound containing the following structural formula (I) and a binding protein, with a myeloma-origin cell line, obtaining monoclonal antibody-producing cells by cloning, and then purifying and acquiring the monoclonal antibody produced by these cells into the culture supernatant 

Peptide fructose and protein conjugate with the same

A method of the present invention is a fluorescence polarization method at multiple wavelengths for analyzing two or more different assay-objects in a sample. The method includes the steps of: (a) providing two or more different fluorescent-labeled substances, each being a substance which is capable of specifically binding to respective one of the assay-objects and is covalently bound to a fluorochrome, wherein the fluorochromes of the fluorescent-labeled substances are different from one another; (b) allowing the fluorescent-labeled substances to bind to the two or more different assay-objects, respectively; and (c) measuring a change in the degree of fluorescence polarization which has taken place in each of the fluorescent-labeled substances by its binding to one of the assay-objects.

Growing community of inventors

Ehime, Japan

Fumihisa Kitawaki