The Texas A&M University System

University of Florida Research Foundation, Incorporated

Fuller Warren Bazer

Jorge A Piedrahita

Howard Marcellus Johnson

Carol H Pontzer

Gino Van Heeke

Troy Lee Ott

Chang-Kyu Lee

Regina Weaks

Provided are methods and compositions for increasing the efficiency of nuclear transfer using apoptosis inhibitors, and for the production of transgenic and non-transgenic mammals from cultured cells or cell lines. Methods for cloning mammals, and for producing transgenic and chimeric mammalian tissues and mammals, and chimeric cell lines are also provided.

Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors

Disclosed are methods for the isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, methods for transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated by the present invention is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.

Methods comprising apoptosis inhibitors for the generation of transgenic pigs

Cells and methods for the generation of transgenic pigs

The invention provides antitumor therapeutic methods employing bovine or ovine interferon-tau (IFN-.tau.) proteins and polypeptides. The IFN-.tau. proteins exhibit the antiviral and antiproliferative properties characteristic of type I interferons. An advantage of the invention is that IFN-.tau. has essentially no cytotoxic effects on treated cells as does, for example, IFN-.alpha..

Antitumor therapy using ovine or bovine interferon-tau

The invention provides antiviral therapeutic methods employing bovine or ovine interferon-tau (IFN-.tau.) proteins and polypeptides. The IFN-.tau. proteins exhibit the antiviral and antiproliferative properties characteristic of type I interferons. An advantage of the invention is that IFN-.tau. has essentially no cytotoxic effects on treated cells as does, for example, IFN-.alpha..

Growing community of inventors

College Station, TX, United States of America

Fuller Warren Bazer