Nanjing Forestry University

Fuliang Cao

Shilong Yang

Li Xu

Fangfang Fu

Xiaoming Yang

Meiling Ming

Wei Xu

Pingjun Xu

Liangjiao Xue

Guibin Wang

Anqi Zhao

Awuschel-related homeobox 3A (GbWOX3A) gene and an application thereof in plant tissue culture are provided. The GbWOX3A gene is derived from aembryo, the gene sequence of the GbWOX3A gene is SEQ ID NO: 1, and the amino acid sequence encoded by the GbWOX3A gene is SEQ ID NO: 2. An expression of the GbWOX3A gene in developing embryo ofand regenerating a callus has specificity, and transgenic function verification experiments prove that overexpression of the GbWOX3A gene can promote regeneration of adventitious buds during the plant tissue culture. Therefore, the GbWOX3A gene has important theoretical significance and application value in the plant tissue culture, and especially plays an important role in improving the regeneration efficiency of gymnosperms.

GBWOX3A gene and application thereof in plant tissue culture

A method for preparing a biflavone-iron complex, includes using the amentoflavone as the ligand and the ferric ion as the central ion, the amentoflavone-iron complex is obtained by reaction, and the structure of the complex is characterized by infrared spectroscopy, UV-vis absorption spectroscopy and high resolution mass spectrometry. Meanwhile, the antitumor and antioxidant activities of the amentoflavone-iron complex are studied in the present invention. MTT method shows that the amentoflavone-iron complex has a relatively good antitumor activity, and the antitumor activity thereof is stronger than that of the amentoflavone. Pyrogallol auto-oxidation method and ABTS method show that the antioxidative activity of the amentoflavone-iron complex is stronger than that of the amentoflavone itself.

Biflavone-iron complex, preparation method and application thereof

The present invention discloses a biflavone-zinc complex, a preparation method and application thereof. In the present invention, using the amentoflavone as the ligand and the zinc ion as the central ion, the amentoflavone-zinc complex is obtained by reaction, and the structure of the complex is characterized by infrared spectroscopy, UV-vis absorption spectroscopy and high resolution mass spectrometry. Meanwhile, the antitumor and antioxidant activities of the amentoflavone-zinc complex are studied in the present invention. MTT method shows that the amentoflavone-zinc complex has better antitumor activity, and the antitumor activity thereof is stronger than that of the amentoflavone. Pyrogallol auto-oxidation method and ABTS method show that the antioxidative activity of the amentoflavone-zinc complex is stronger than that of the amentoflavone itself. The formation of the flavonoid-zinc complex enhances the antitumor and anti-oxidation activities of the amentoflavone, and is expected to be used in the development of drugs.

Biflavone-zinc complex, preparation method and application thereof

A method for preparing a biflavone-cobalt complex includes using the amentoflavone as the ligand and the cobalt ion as the central ion, the amentoflavone-cobalt complex is obtained by reaction, and the structure of the complex is characterized by infrared spectroscopy, UV-vis absorption spectroscopy and high resolution mass spectrometry. Meanwhile, the antitumor and antioxidant activities of the amentoflavone-cobalt complex are studied in the present invention. MTT method shows that the amentoflavone-cobalt complex has better antitumor activity, and the antitumor activity thereof is stronger than that of the amentoflavone. Pyrogallol auto-oxidation method, ABTS method and FRAP method show that the antioxidan activity of the amentoflavone-cobalt complex is stronger than that of the amentoflavone itself. The formation of the flavonoid-cobalt complex enhances the antitumor and anti-oxidation activities of the amentoflavone, and is expected to be used in the development of drugs.

Biflavone-cobalt complex, preparation method and application thereof

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Nanjing, China

Fuliang Cao