Mitsubishi Rayon Company, Limited

Mitsubishi Chemical Corporation

Nitto Chemical Industry Co., Ltd.

The University of Tokyo

Other

University of California

Toyama Prefectural University

Nitto Chemical Co., Ltd.

Fujio Yu

Wataru Mizunashi

Eiji Sato

Tetsuji Nakamura

Yasuhisa Asano

Eiji Nakajima

Fumiaki Watanabe

Takanori Akiyama

Michiko Yamazaki

Tetsuo Nagano

Yoshihiro Hashimoto

Yasuteru Urano

Miki Sakai

Dai Ujihara

Tomoe Ohta

Takashi Akita

Ichiro Watanabe

Teruhiko Beppu

Toshinori Sumi

Sueharu Horinouchi

Teruta Ishimaru

Kei Murase

Kevin V Solomon

Takayuki Makino

Makoto Nishiyama

Kozo Murao

Michelle O'Malley

Haruko Miyauchi

Mami Kato

Osamu Maehara

Toshitaka Uragaki

Kenji Oki

Wataru Fujii

Nobuko Oogami

Atsushi Takahashi

Tadanobu Ikeda

Chiho Ito

Takeharu Morishita

Yurie Mizumura

Fumihiro Motojima

Toshio Fujita

As a method for producing a 3-hydroxyisobutyric acid ester using a biocatalyst, a method for producing a 3-hydroxyisobutyric acid ester, including a step of allowing an alcohol or phenol to act on 3-hydroxyisobutyryl-CoA in the presence of alcohol acyltransferase to produce a 3-hydroxyisobutyric acid ester is provided.

Method for producing 3-hydroxyisobutyric acid ester and methacrylic ester

The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genusor

Method for producing methacrylyl-CoA

It is intended to provide an efficient method for expressing a protein of interest as an active soluble recombinant protein in a transformant. The present invention provides a method for producing an enzyme having improved activity per recombinant as compared with a reference form, comprising the steps of: (1) preparing recombinants each expressing a mutant having an amino acid sequence derived from the amino acid sequence of the reference form by the substitution of one or more cysteine residues by other amino acid residues; (2) selecting a plurality of mutants that exhibit 50% or more activity per recombinant relative to 100% activity of the reference form; and (3) expressing a mutant in which corresponding amino acid residues are substituted at two or more positions among the respective positions of the substituted amino acid residues of the mutants selected in step (2).

Method for producing mutant enzyme, and mutant alcohol acyltransferase

Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step for treating methacrylyl-CoA with an alcohol or phenol in the presence of an alcohol acyltransferase to synthesize the methacrylic acid ester. According to this production method, a methacrylic acid ester can be efficiently produced while largely reducing energy, resource and environmental load, compared with the conventional chemical production processes.

Method for producing methacrylic acid ester

The invention relates to an engineered eukaryotic microorganism into which a gene encoding an acyl-CoA dehydrogenase is introduced and a method for producing methacrylic acid esters such as MMA and MMA-CoA and precursors thereof using the microorganism.

Biological production of methyl methacrylate

Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step of reacting an alcohol or a phenol with methacrylyl-CoA in the presence of an alcohol acyltransferase originated from a plant selected from the group consisting of a plant belonging to the genus, a plant belonging to the genus, a plant belonging to the genus, a plant belonging to the genus, a plant belonging to the genusand a plant belonging to the genusto thereby synthesize the methacrylic acid ester.

Method for producing methacrylic acid ester and novel methacrylic acid ester synthetase

To provide a method for directly and efficiently producing methacrylic acid in a single step from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials. Further provided is a method for producing methacrylic acid using microbes having the ability to produce methacrylic acid, from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials, as a carbon source and/or energy source. The method for producing methacrylic acid enables methacrylic acid to be safely and easily produced from biomass, without using petroleum-derived raw materials, by utilizing microbes having the ability to produce methacrylic acid.

Method for producing methacrylic acid and/or ester thereof

The object of the invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme and compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which includes the compound.

Fluorescent substrate for detection of enzymatic activity of nitrile-related enzyme

The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.

An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

Hydroxynitrile lyase

Substitutional variants of hydroxynitrile lyase with high specific activity and methods of use

The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

Heat-resistant nitrile hydratase

Growing community of inventors

Kanagawa, Japan

Fujio Yu