Boehringer Mannheim Gmbh

Fritz Stahler

Wolfgang Gruber

Sigmar Klose

Gunter Weimann

Erich Bernt

Erich Haid

August Wilhelm Wahlefeld

Hans-Ralf Linke

Karl Wulff

Hans-Georg Batz

Helmut Lenz

Hans Lange

Winfried Albert

Gerd Kleinhammer

Gerhard Michal

Wolfgang Kleemann

Dieter Hundt

Marianne Sedelmeyer

Peter Roschlau

Gerlinde Deutsch

The present invention provides a process for carrying out an analytical determination by mixing and incubating a sample solution with at least one reagent and measuring a parameter in the reaction mixture, the sample solution being transported from an application point to a measurement point, wherein a sample solution is first transported to a soluble dry reagent, with at least partial dissolving of the latter, and then further transported to a measurement point, the transport taking place by two different forces, whereby, at least on a part of the transport path, it is brought about by a surface force acting on the solution as a first force, which, for the regulation of the transport velocity or transport direction, is superimposed by a centrifugal force and/or pressure force as a second force which, depending upon which transport state of the fluid is to be adjusted, is made greater or smaller than the first force.

Process for carrying out analytical determinations and means for

A process for carrying out an analytical determination by mixing and incubating a sample solution with at least one reagent and measuring a parameter in the reaction mixture, the sample solution being transported from an application point to a measurement point, wherein a sample solution is first transported to a soluble dry reagent, with at least partial dissolving of the latter, and then further transported to a measurement point, the transport taking place by two different forces, whereby, at least on a part of the transport path, it is brought about by a surface force acting on the solution as a first force, which, for the regulation of the transport velocity or transport direction, is superimposed by a centrifugal force and/or pressure force as a second force which, depending upon which transport state of the fluid is to be adjusted, is made greater or smaller than the first force.

The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent and optically measuring a parameter in the incubated reaction mixture. The mixing, incubating and measuring are carried out during the action of a centrifugal force which forces the solution through a plurality of interconnected small hollow spaces having flow resistance so adapted with regard to one another as to mix the reaction components. Incubation may also take place before the reaction solution passes from the plurality of interconnected small hollow spaces into a measuring chamber in which the measurement is carried out.

Method for carrying out analytical determinations

A method for the determination of the thyroxine-binding index in serum, which method comprises mixing the serum sample to be determined with a defined amount of thyroxine and a defined amount of a determinable enzyme covalently bound to thyroxine, contacting the resulting solution with anti-thyroxine antibodies present in the solid phase, separating the liquid phase from the solid phase, and measuring the activity of said determinable enzyme in one of the phases as a measure of the tyroxine-binding index in said serum sample.

Determination of the thyroxine-binding index in serum

Process for the preparation of reactive, couplable derivatives of the thyroid hormones 3,3',5-triiodothyronine (T.sub.3) and 3,3',5,5'-tetraiodothyronine (T.sub.4) by reaction with reactive carboxylic acid derivatives, which process comprises sylylating all the functional groups of the thyroid hormone by reaction with a reactive triorganosylyl derivative and then reacting the persylylated derivative of the thyroid hormone thus obtained with an activated carboxylic acid derivative.

Process for the preparation of reactive, couplable derivatives of the

Creatine kinase is determined by the reaction of creatine phosphate with adenosine diphosphate with the formation of adenosine triphosphate, reaction of the latter with luciferin and oxygen in the presence of luciferase and diadenosine pentaphosphate with the formation of oxyluciferin and adenosine monophosphate, and measurement of the light thereupon emitted, the reaction being performed with a saturated concentration of adenosine diphosphate and substrate, in the presence of 1 to 10 millimoles per liter of AMP at pH values of 5.8 to 7.5, with at least 50 units of luciferase per test. It is desirable to operate in the presence of diadenosine pentaphosphate or sodium fluoride and a sequestering agent.

Method and reagent for the determination of creatine kinase

A method for the quantitative determination of an oxidized pyridine co-enzyme which comprises reacting a sample containing oxidized pyridine co-enzyme in the presence of its specific alcohol dehydrogenase with an aliphatic alcohol of 8 to 16 carbon atoms, oxidizing the aldehyde formed with reduced flavine mononucleotide and oxygen in the presence of luciferase and of the particular FMN-reductase which is independent of the co-enzyme to be determined and of the reduced substrate co-enzyme of the latter, and measuring the resulting light emission as a measure of the initial oxidized pyridine co-enzyme content.

Method and composition for determining an oxidized pyridine co-enzyme

Control serum containing a defined content of creatine kinase comprising exogeneous creatine kinase in the form of swine serum and optionally additionally comprising human serum, other animal serum or serum albumin; and/or other enzyme substrates and metabolites occurring in serum.

Creatine kinase control serum

.gamma.-Glutamyl-4-nitroanilide compounds of the formula ##STR1## wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyl-transpeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycylglycine and determining the liberation of the resulting p-nitro-aniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

.gamma.-Glutamyl-4-nitroanilide compounds

.gamma.-Glutamyl-4-nitroanilide compounds of the formula ##SPC1## Wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyltranspeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycyl-glycine and determining the liberation of the resulting p-nitroaniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

.gamma.-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING

.gamma.-Glutamyl-4-nitroanilide compounds of the formula ##SPC1## Wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyltranspeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycylglycine and determining the liberation of the resulting p-nitroaniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

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Tutzing, Germany

Fritz Stahler