Girindus Ag

Centre National De La Recherche Scientifique

University of Montpellier Ii

Fritz Link

Meinolf Lange

Jean-Louis Imbach

Jean-Jacques Vasseur

Albert Meyer

Olaf Groessel

Andreas Schoenberger

Andreas Hohlfeld

Ilaria Adamo

Andreas Schonberger

Meinhof Lange

Nasir Hayat

Christiane Neuhaus

Bernhard Westermann

Christina Kirchhoff

Olaf Grössel

Andreas Schönberger

Nadja Omelcenko

A method for purifying a protected oligonucleotide comprising the steps of:

Purification of oligonucleotides

A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3'-protected compound of formula: with a nucleotide derivative having a 5&#x2032;-protection group, or reacting a 5&#x2032;-protected compound of formula with a nucleotide derivative having a 3&#x2032;-protection group.

Method for preparing oligonucleotides

A process for the synthesis of compounds of general formulas comprising the step of reacting in the presence of wherein:

Method for the production of a-(alpha-hydroxyalkyl)-1,3 dioxan-5-ones

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Bensberg, Germany

Fritz Link