Hewlett-packard Company

Agilent Technologies, Inc.

Fritz Bek

Klaus E Witt

Sally A Swedberg

Laurie S Mittelstadt

Patrick Kaltenbach

Volker Brombacher

Martin Kratzmeier

Xiaodan Tian

A kit for optically detecting proteins, in particular lipoproteins, in a sample, the kit comprising: a chip for performing a separation of the proteins, in particular of the lipoproteins, wherein the chip comprises at least one well for receiving the sample, and a separation channel coupled to the at least one well and being adapted for separating different compounds and a marker dye which contains a polymethine of the general formula (I) wherein Z is a substituted derivative of benzooxazole, benzodiazole, 2,3,3-trimethylindolinine, 2,3,3-trimethyl-4,5-benzo-3H-indolinine, 3- and 4-picoline, lipidine, chinadine and 9-methylacridine derivatives with the general formula IIa or IIb or IIc and wherein X is selected from the group consisting of O, S, Si, N-alkyl and C(alkyl), n is 0, 1 , 2 or 3, Rto Rare independently selected from the group consisting of hydrogen, alkyl, alkoxy, cycloalkyl, linear or branched alkenyl, cycloalkenyl, aryl, heteroaryl, heterocycle, hydroxy, carboxy, amine, alkyl-substituted amine and cyclic amine and/or two or more fragments in ortho-position to each other, for example Rand Ror R, Rand R, together form another cycloalkyl ring or ring system, heterocyclic ring or ring system, heteroaryl ring system or aromatic ring or ring system. 

Protein analysis using a polymethine marker dye

Low intensity light is incident upon a photodiode whose output is coupled to an integrator. The output of the integrator is coupled to an input of an A/D converter, whose output is coupled to a microprocessor, whose output is coupled to a filter. A second output of the microprocessor is coupled to a gate electrode of a FET to provide a reset signal to the FET to reset the integrator. The microprocessor compares the digital samples of the integrated signal from the A/D converter and, firstly, generates the reset signal if a sample is beyond a set limit, and, secondly, calculates delta values between adjacent samples and interpolates the delta values for the reset periods so as to provide a continuous data stream which can be filtered by a filter matched to the form of the original signal.

Method and apparatus for measuring a low power signal

Miniaturized planar column devices for use in a liquid phase separation apparatus are described. The devices include microstructures that have been fabricated by laser ablation in a variety of novel support substrates. Devices formed according to the methods of the invention include associated laser-ablated features required for function, such as on-device reservoirs or makeup flow compartments, analyte detection means and sample injection means. The miniaturized columns can be used in an apparatus intended for analysis of either small and/or macromolecular solutes in the liquid phase which employs chromatographic, electrophoretic or electrochromatographic separation means. The apparatus can include a variety of optional injection means, manifolds, keeper means, post column collection means, and combinations thereof.

Miniaturized planar columns for use in a liquid phase separation apparatus

Miniaturized planar column devices are described for use in liquid phase analysis, the devices comprising microstructures fabricated by laser ablation in a variety of novel support substrates. Devices formed according to the invention include associated laser-ablated features required for function, such as analyte detection means and fluid communication means. Miniaturized columns constructed under the invention find use in any analysis system performed on either small and/or macromolecular solutes in the liquid phase and may employ chromatographic, electrophoretic, electrochromatographic separation means, or any combination thereof.

Miniaturized planar columns in novel support media for liquid phase

Miniaturized planar columns for use in a liquid phase separation

Method for making miniaturized planar columns in novel support media for

A method and apparatus for mixing liquids using electroosmotic flow. Multiple capillaries (100, 104, 106) or microchip channels meet at a common junction (102). One capillary (100) is used for mixed liquids and each of the remaining capillaries (104, 106) are used for supplying a liquid (112, 114) to be mixed. Each of the supply capillaries (104, 106) has a free end that is immersed into a vial (108, 110) of liquid (112, 114). A first power supply terminal (112) is attached to a free end of the mixed liquid capillary (100). The liquid in each vial has an electrode that is switchably connected to a second power supply terminal. Closing a switch (116, 118) causes liquid to flow from a vial through the common junction and through the mixed liquid capillary. Each switch is independent and each may be closed continuously, switched at a constant duty cycle, or switched at a variable duty cycle. As a result, continuous mixing or variable ratio mixing may be achieved.

Mixing liquids using electroosmotic flow

A miniaturized column analytical apparatus having a miniaturized column device containing a body with an elongate separation compartment is provided. Two or more sets of spaced apart antennas are positioned along the elongate separation compartment. The elongate separation compartment has first and second opposing sides along its elongate dimension. Each set of antenna contains a plurality of antennas. One antenna from each set is associated with at least one antenna from each of the other sets to form repeating sequences of antennas along the separation compartment on the opposing sides of the separation compartment. Each set of the antennas is associated with a different oscillating electrical potential to provide a plurality of oscillating electric fields along the elongate separation compartment to draw a target substance along the elongate separation compartment toward an exit end of the separation compartment. The detector can detect a target substance passing out of the elongate separation compartment.

Low voltage miniaturized column analytical apparatus and method

Miniaturized planar column devices and miniaturized total analysis systems for liquid phase analysis are disclosed. The systems include a temperature control device for regulating temperature, preferably a thermoelectric device comprising Peltier elements.

Use of temperature control devices in miniaturized planar column devices

A miniaturized total analysis system ('.mu.-TAS') comprising a miniaturized planar column device is described for use in liquid phase analysis. The .mu.-TAS comprises microstructures fabricated by laser ablation in a variety of novel support substrates. The .mu.-TAS includes associated laser-ablated features required for integrated sample analysis, such as analyte detection means and fluid communication means. .mu.-TAS constructed according to the invention is useful in any analysis system for detecting and analyzing small and/or macromolecular solutes in the liquid phase and may employ chromatographic separation means, electrophoretic separation means, electrochromatographic separation means, or combinations thereof.

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Waldbronn, Germany

Fritz Bek