Biomerieux, Inc.

Université De Caen Normandie

Frédéric Fabis

Ali Laayoun

Alain Laurent

Sylvain Ursuegui

Thomas Cailly

The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.

Reagents for reversibly protecting biological molecules

The present invention relates to a method of functionalization of at least one ribonucleic acid (RNA) molecule, contained in a liquid sample, which includes the following steps: a) providing at least: one binding molecule consisting of an aza-isatoic anhydride or a derivative thereof, one group of interest, and one linkage joining the binding molecule to the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group carried: in position 2' of the ribose of one of the RNA nucleotides, and/or in position(s) 2&#x2032; and/or 3&#x2032; of the ribose of the nucleotide at the terminal 3&#x2032; end of the RNA, and obtaining an aza-anthranilate that joins, by means of the linkage, the RNA to the group of interest.

Methods of functionalization and reagents used in such methods using an aza-isatoic anhydride or a derivative thereof, biological molecules thus treated and kits

Growing community of inventors

Mathieu, France

Frédéric Fabis