Agilent Technologies, Inc.

Fredrik Roos

Henrik Johansson

Magnus Isaksson

Olle (Olof) Ericsson

Simon Fredriksson

Mats Nilsson

A method for amplifying a target nucleic acid is disclosed, which includes: (a) fragmenting a nucleic acid sample to create a target fragment comprising a target nucleic acid and two probe-complementary portions; (b) contacting said fragmented nucleic acid sample with a probe comprising two target fragment-complementary portions complementary to the probe-complementary portions of the target fragment; (c) rendering the fragmented nucleic acid sample single-stranded; (d) allowing the probe-complementary portions to hybridise with the target-fragment complementary portions; (e) if the probe in step (b) is not immobilised, immobilising the probe-target fragment hybrid on a solid phase via immobilisation moiety; (f) separating non-immobilised nucleic acid fragments from the solid phase; (g) contacting the solid phase with a ligase to ligate ligatable 5' and 3&#x2032; ends of the target fragment whereby the target fragment is circularized; and (h) amplifying said circularized target fragment.

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Loveland, CO, United States of America

Fredrik Roos