Janssen Pharmaceutica Nv

Ortho-mcneil Pharmaceutical Corp.

Intrexon Corporation

Fredrik Kamme

Manfred Koller

Jessica Y Zhu

Bernhard H Meurers

Dmitri Talantov

Jingxue Yu

Gustaf Angelborg

James Linton

The present invention provides a new method for producing amplified RNA from a selected set of cells. This method combines and utilizes known methods in a novel way to produce the gene expression profiles. The methods of the present invention utilize thermostable DNA polymerases and RNase H to produce high fidelity second strand synthesis of nucleic acids from selected cells in a highly efficient mariner and in substantially reduced time.

Method for generating amplified RNA

To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or tris-(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an immunostaining assay and other histochemical methods.

Preservation of RNA in a biological sample

Growing community of inventors

San Diego, CA, United States of America

Fredrik Kamme