Minnesota Mining and Manufacturing Company

3m Innovative Properties Company

Frederick W Hyde

Steven M Heilmann

Louis C Haddad

Gary J Drtina

Dean M Moren

Todd W Johnson

Philip D Eitzman

Robert A Pranis

Michael G Williams

Jerald K Rassmussen

Insoluble supports are prepared which possess high surface areas and efficiently dispersed isocyanate groups. These reactive supports are useful for covalently binding proteins which preferably are enzymes and provide catalysts for conducting organic reactions.

Crosslinked isocyanate functional polymer supports

Crosslinked isocyanate-functional polymer supports

Insolubilized enzyme particulates are located on the upstream surface of a filtration layer of a filtering element which can be used in a cartridge filter. Employed in a recycling flow reaction system at relatively high flux rates, the cartridges are useful for conducting catalyzed chemical reactions of dissolved solutes, for example, esterifications, isomerizations, oxidations, reductions, and cyclizations.

Cartridge filters with insoluble enzyme particulates contained thereon

The invention provides a method of separating a biomacromolecule which comprises the steps of providing a separation system including a filter element which comprises a composite filtration medium, the composite filtration medium comprising a filtration layer on the upstream surface of which are located insoluble stationary phase particulates, the particulates being capable of binding to a biomacromolecule or class of biomacromolecules, a reservoir containing a solution mixture comprising at least one biomacromolecule as solute, and a pump and associated tubing to form a closed loop assembly, and recirculation pumping the solution mixture through the filter cartridge so as to bind the at least one biomacromolecule to the stationary phase particulate so as to form a biomacromolecule:stationary phase particulate product. An eluting solution can be pumped through the closed loop assembly which is capable of reversing the biomacromolecule:stationary phase particulate product binding interaction so as to liberate the biomacromolecule.

Method of isolating and purifying a biomacromolecule

Growing community of inventors

New Brighton, MN, United States of America

Frederick W Hyde