Fred Hutchinson Cancer Center

Frederick Cross

James M Roberts

Andrew C Koff

Motoaki Ohtsubo

Methods are provided for determining in biological material the presence or amount human cyclin E polypeptide and/or cyclin E:cell division kinase complexes.

Immunoassays for human cyclin E

Nucleic acid molecules capable of hybridizing under stringent conditions to the nucleotide sequence residing between positions 1 and 1185 of the human cyclin E cDNA sequence shown in FIG. 2. Polypeptides encoded by such nucleic acid molecules, and immunologic binding partners directed to such polypeptides.

Human cyclin E

Human cyclin E polypeptides

Whole cell or cell-free test systems for screening and identifying compounds that modulate or alter Cyclin E activity. Whole cell assays measure the G1 phase of cells in the presence or absence of the test compound. Additional whole cell or cell-free assays measure binding of cyclin E to a cell division kinase, measure cyclin E activity directly, or measure the cell division kinase activity directly in the presence or absence of the test compound.

Assays for compounds that modulate or alter cyclin E activity

Nucleic acid molecules capable of hybridizing under stringent conditions to the nucleotide sequence residing between positions 1 and 1185 of the human cyclin E cDNA sequence shown in FIG. 2 . Polypeptides encoded by such nucleic acid molecules, and immunologic binding partners directed to such polypeptides.

Growing community of inventors

New York, NY, United States of America

Frederick Cross