Harvard College

Other

Frederic Vigneault

George M Church

Richard C Terry

Francois Vigneault

Kalim U Mir

François Vigneault

Methods of detecting, probing, mapping and directed sequencing of target nucleic acids are provided using a guide RNA and a Cas9 protein. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the guide RNA includes a 3' tail sequence that can hybridize to a probe are provided. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the complex is physically detected are provided.

RNA-guided systems for probing and mapping of nucleic acids

A method of sequencing nucleic acids is provided using DNA origami as a barcode for a nucleic acid probe.

Spatial sequencing of nucleic acids using DNA origami probes

A method of sequencing nucleic acids is provided using sequencing by ligation and/or sequencing by hybridization.

Sequencing by structure assembly

A method of sequencing nucleic acids by probe hybridization and/or ligation is provided using DNA origami as a barcode for a nucleic acid probe.

Growing community of inventors

Ashland, MA, United States of America

Frederic Vigneault