Emd Millipore Corporation

Frederic Marc

Gael Waiche

Sebastien Ribault

Luc Felden

Jean-Jacques Richert

The invention relates to a growth medium allying a fluorogenic substrate and a pH-sensitive fluorophore, in particular the combination of 4-methylumbelliferone (4-MU) and derivatives of fluorescein. This growth medium is used for the detection by fluorescence of microorganisms by coupling the fluorescence measurements relating to the pH-sensitive fluorophor and the fluorescence measurements relating to the activation of the fluorogenic substrate(s) by the microorganisms.

Growth medium for the detection of microorganisms by fluorescence allying a fluorogenic substrate and a pH-sensitive fluorophore

A device () for separating a membrane () to analyze from a support (), the membrane being mounted in a frame (), itself mounted on a support, having a body () comprising a longitudinal recess () for translational reception of the assembly constituted by the frame mounted on the support, the recess having a first end () and a second end () and longitudinal sides (A,B,) facing each other between said two ends, the device having bearing means () for a first member of the frame/support group between the first and the second end of the recess, which are adapted to serve as a bearing for the first member of the frame/support group once the assembly is disposed in the recess, each of the sides having a ramp (A,B,) adapted to engage a gripping member () of the second member of the frame/support group when the assembly is translationally moved from the first to the second end, said ramps being adapted to separate the support from the frame during said translational movement by bearing on said gripping member of the second member of the frame/support group, the first member of the frame/support group then bearing on the bearing means, such that the frame and the support are separated once the translational movement has arrived at the second end.

Device for separating a membrane from a support

The present invention relates to a method using a composition for permeabilizing microorganism walls for counting and detecting in a targeted manner the microorganisms on a membrane. The invention also relates to a kit and to probes that are suitable for carrying out the method.

Growing community of inventors

Itterswiller, France

Frederic Marc