Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Biomerieux, Inc.

Commissariat a L'energie Atomique

Biomerieux Sa

Frédéric Mallard

Mathieu Dupoy

Armelle Novelli-Rousseau

Patrick Pouteau

Laurent Drazek

Frédéric Pinston

Pierre Joly

Pierre Marcoux

Florence Ricoul

Quentin Josso

Jean-Pierre Moy

Frederic Ginot

Raymond Campagnolo

Alice Douet

Frédéric Ginot

Geneviève Bossy

Armelle Novelli Rousseau

Olivier Figadere

Maryse Guicherd

Isabelle Espagnon

Fabien Romanens

Olivier Gal

Raphaël Mathey

The invention concerns a method for determining the reaction of at least one bacterium of interest to its exposure to an antibiotic implementing a Raman spectroscopy analysis comprising the following steps:

Method for determining the reaction of a microorganism to its exposure to a chemical compound

A method for observing a sample is provided. The method includes the steps of: a) providing a volume (G, V) of a substantially transparent medium having a first refractive index (n) and having a first surface (S) that is convex, rounded, or faceted, and a second substantially planar surface (S), the first surface separating the volume from a surrounding medium (MA) having a second refractive index (n) that is lower than the first index, the sample to be observed being contained inside the volume or being deposited on the first surface; b) observing the sample through the second substantially planar surface; and c) determining the presence of a substance or an object in the sample by detection during step b), a ring of light that correlates with the volume.

Method for observing a sample

The invention relates to a method for detecting clusters of biological particles () on a surface (), comprising steps that involve: a. determining (E) a topographical representation () of said surface; and b. detecting (E, E), on said topographical representation, at least one contour defining a region that is likely to correspond to a cluster of biological particles.

Method for detecting clusters of biological particles

The disclosure relates to a biological analysis device including:

Device for bioassays with integrated detector

Analysis chip of at least one analyte, said chip comprising at least one analysis spot for recognition and immobilization specific to the analyte; and a reference range (G) comprising several reference spots each arranged on said chip in a defined manner and independently of one another, each reference spot of this range comprising, immobilized on its surface and in a defined proportion P that is different and known for each spot relative to the other reference spots of said range: a probe reference molecule (PRM) permitting recognition and hybridization specifically to a defined target reference molecule (TRM), and/or an inert oligonucleotide molecule (IM) incapable of recognition and hybridization with said PRM, these molecules both being unable to immobilize said analyte or analytes. The method of the invention comprises the use of said chip with its reference range for analysis of said, at least one, analyte.

Analysis chip with reference range, kits and methods of analysis

Growing community of inventors

Voreppe, France

Frédéric Mallard