Bertin & Cie

Framatome Gmbh

Frederic Dufau

Jean Hache

Daniel Cohen

Frederic Ginot

Bernard Dutertre

Dominique Cognard

Dominique Duval

Isabelle Geahel

Patrick Binot

Jean-Louis Bernou

Agnes Marcadet-Troton

A process of separating plasmids and cosmids from bacterial cells in which they are contained, comprising adding cultured bacterial cells separated from a culture medium to an inclusion gel-forming substance; lysing the bacterial cells; solubilizing the thus lysed bacterial cells; solidifying the inclusion gel-forming substance to form a gel either before or after the step of solubilizing; and applying an electric field to the thus formed gel sufficient to cause electrophoretic migration of the plasmids and cosmids and isolation of the plasmids and cosmids. Also disclosed is a device for carrying out the process.

Installation for obtaining plasmids and cosmids

Cell culture device

Method and apparatus for high speed regulation of a wall temperature

The filtration apparatus comprises a bundle of porous filtering elements (12) which are disposed over a certain length of a casing (2) and over a substantial part of its cross-section, and at least one assembly of ultrasonic transducers (15) disposed in at least one cross-sectional plane of the casing regularly spaced at its periphery. Each of the transducers is placed within an opening passing through the casing in a direction perpendicular to the axis of the casing. Ultrasonic waves are emitted within the casing in the direction of the bundle of filtering elements in a zone delimited by a cone, the axis of which extends in a direction perpendicular to the filtering elements and the vertex of which is located in the vicinity of the wall of the casing. The particles torn out by the ultrasonic waves from the filtering elements are recovered by an evacuation conduit (8). The invention is applicable, in particular, to the treatment of fluid used in the nuclear industry, and of other dangerous products.

Filtration apparatus comprising an ultrasonic cleaning device and

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La Celle Saint Cloud, France

Frederic Dufau