Roche Diagnostics Operations, Inc.

Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Other

F. Hoffmann-la Roche Ag

Frederic Donie

Barbara Upmeier

Michael Wiedmann

Dittmar Schlieper

Herbert Von Der Eltz

Hans-Peter Josel

Marie-Ange Buyse

Rupert Herrmann

Eva Hoess

Elke Faatz

Erasmus Huber

Herbert Andres

Peter Sluka

Helmut Lill

Rudolf Vogel

Christine Markert-Hahn

Eric Saman

Rosemarie Kientsch-Engel

Ralf Bollhagen

Rainer Wehner

Ursula Klause

Andreas Finke

Rupert Hermann

Beatus Ofenloch-H&auml;hnle

Jean-Marie Freyssinet

Benedicte Antoni

Martina Bronold

Methods for determining an analyte in a sample by immunoassay in a one-step format without performing washing steps are described. The method includes a first analyte-specific receptor that contains at least two binding sites for the analyte, and a second analyte-specific receptor that selectively binds to an aggregate arrangement of at least two analyte molecules bound to the first receptor.

Method for detection of specific immunoglobulin class G antibodies

The invention relates to a process for the production of a biomolecule-linker conjugate of uniform stochiometry. It especially relates to a conjugate consisting of a biomolecule of a molecular weight between 5 kD and 500 kD and a hydrophilic linker molecule said linker having a molecular weight between 1 and 15 kD and between 4 and 60 charged residues, characterized in that said conjugate comprises at least one biomolecule-linker product of uniform stoichiometry in a pre-selected amount.

Conjugates of defined stoichiometry

The invention concerns the use of intramolecularly, covalently cross-linked proteins and covalently cross-linked reverse transcriptase from HIV as immunological binding partners in immunoassays. It also concerns immunological test procedures for detecting an analyte in a sample in which intramolecularly, covalently cross-linked proteins are used as binding partners, and it further concerns intramolecularly, covalently cross-linked reverse transcriptase from HIV and a method for producing this reverse transcriptase.

Use of intramolecularly, covalently cross-linked proteins as binding partners in immunoassays

The present invention relates to determining the prethrombotic state, in particular determining an amount or presence of circulating microparticles and/or stimulated procoagulant cells.

Method for the determination of the prethrombotic state

The invention concerns a process for diagnosis of an HIV infection by means of an immunoassay using the specific detection of the p24 antigen of HIV1, HIV1-Sub0 and/or the p26 antigen of HIV2, at least one antibody against the env region of HIV1, HIV1-Sub0 and/or of HIV2 and at least one antibody against the pol and/or gag region of HIV1, HIV1-Sub0 and/or HIV2, reagent kits and test strips suitable for diagnostic procedure as well as monoclonal antibodies against p24 and their use.

Method for simultaneous detection of HIV antigens and HIV antibodies

The invention concerns a method for the determination of an analyte in which rheumatoid factors or rheumatoid-factor-like substances are added as an interference reducing reagent to reduce of avoid a hook effect. The invention in addition concerns suitable reagent kits for carrying out the method.

Interference reduction by rheumatoid factors

The invention addresses new photo-activatable biotin derivatives having the formula I ##STR1## wherein R=�(CH.sub.2).sub.n --O.sub.m !.sub.q --�(CH.sub.2).sub.r --O.sub.s !.sub.t --(CH.sub.2).sub.p

Photo-activatable biotin derivatives and their use to reduce

Interference eliminating agent for application in immunoassays

Growing community of inventors

Penzberg, Germany

Frederic Donie