Carl Zeiss Microimaging Gmbh

Mesa Imaging Ag

Fred Silvester Wouters

Thierry Oggier

Felix Lustenberger

Alessandro Esposito

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique increasingly used in the life sciences during the past decades. An all-solid-state fluorescence-lifetime-imaging microscope () with a simple lock-in imager () for fluorescence lifetime detection is described. The lock-in imager (), originally developed for 3D vision, embeds all the functionalities required for FLIM in a compact system. Its combination with a light-emitting diode () yields a cost-effective and user-friendly FLIM unit for wide-field microscopes. The system is suitable for nanosecond lifetime measurements and achieves video-rate imaging capabilities.

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Gottingen, Germany

Fred Silvester Wouters