Biotechnology Research and Development Corporation

United States of America, As Represented by the Secretary of Agriculture

The United States of America As Represented by the Department of

Other

The United States of America As Represented by the Secretary of

The United States of America As Represented by the Department of Agriculture

Biotechnology and Research and Development Corporation

Fred M Tatum

Robert E Briggs

Robert A Miller

Felicito Guerrero

Adalberto A Perez De Leon

The present invention relates to modified() lktCA gene cluster cassettes, compositions comprising such cassettes, methods of using such cassettes and compositions, and kits comprising such cassettes and compositions. Also described herein are() protective antigens, compositions comprising such antigens, methods of using such antigens and compositions, and kits comprising such antigens and compositions. Also described herein are modifiedlktCA gene cluster cassettes engineered to expressprotective antigens, compositions comprising such cassettes, methods of using such cassettes and compositions, and kits comprising such cassettes and compositions.

vector

The present invention provides novel attenuatedstrains that may be used, in live or killed form, to formulate vaccines that are highly protective againstinfection in bovines, other mammals, and in birds. The present invention also identifies the combination of nanP and hyaC gene mutations as key to the provision of such vaccines. When appropriately formulated, antigenic material of numerous other bovine pathogens may be combined with the live attenuatedstrains, to make effective combination vaccines.

strains and vaccines having hyaC and nanP deletions

This invention relates to novel fusion peptides and immunogenic compositions containing the fusion peptides useful in the control and prevention of tick infestations. The invention also relates to compositions comprising said fusion peptides, methods of vaccination against tick infestation using said fusion peptides and compositions, and kits for use with such compositions and methods.

Southern cattle tick vaccine product

Mycoplasma bovis vaccine product

This disclosure provides attenuatedstrains which can be used to prepare vaccine compositions useful for protection against

Attenuated pasteurella multocida strains

The present invention provides attenuatedstrains that elicit an immune response in animal against, compositions comprising said strains, methods of vaccination against, and kits for use with such methods and compositions. The invention further provides multi-valent vaccines, which provide protective immunity when administered in an effective amount to animals susceptible to 'shipping fever' or bovine respiratory disease.

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Acapsular hyaE deletion mutants ofcan be administered to mammals, particularly ungulates, or birds to provide protective immunity against wild-typee.g., to prevent or reduce the severity of hemorrhagic septicemia or pneumonia in mammals, particularly livestock, ungulates, and companion animals, or fowl cholera in birds, particularly poultry.

Mutants ofprovide excellent safety and efficacy when used as vaccines in ruminants, for example cattle, sheep, and goats, subject to pneumonic pasteurellosis. They can be administered by a variety of routes. Especially preferred is the use in animal feeds. The mutants are not reverting and contain no foreign DNA and no introduced antibiotic resistance genes.

LktA deletion mutant of P. haemolytica

Methylation of DNA can be a critical step in the introduction of DNA into . A methyltransferase has been isolated and molecularly cloned for this purpose. Use of the methyltransferase has allowed construction of defined, attenuated mutants for use as vaccines to protect cattle.

Construction of Pasteurella haemolytica vaccines

Mutants of provide excellent safety and efficacy when used as vaccines in ruminants, for example cattle, sheep, and goats, subject to pneumonic pasteurellosis. They can be administered by a variety of routes. Especially preferred is the use in animal feeds. The mutants are not reverting and contain no foreign DNA and no introduced antibiotic resistance genes.

Temperature sensitive plasmids of P. haemolytica

Mutants of,provide excellent safety and efficacy when used as vaccines in ruminants, for example cattle, sheep, and goats, subject to pneumonic pasteurellosis. They can be administered by a variety of routes. Especially preferred is the use in animal feeds. The mutants are not reverting and contain no foreign DNA and no introduced antibiotic resistance genes.

LKTA deletion mutant of P. haemolytica

Methylation of DNA can be a critical step in the introduction of DNA into P. haemolytica. A methyltransferase has been isolated and molecularly cloned for this purpose. Use of the methyltransferase has allowed construction of defined, attenuated mutants for use as vaccines to protect cattle.

Growing community of inventors

Ames, IA, United States of America

Fred M Tatum