Coulter Electronics, Inc.

Fred L Larsen

Harold R Crews

Stephen L Ledis

James H Carter

Ted Sena

An improved reagent provides combined diluting and lysing of whole blood for use in instruments performing electronic blood cell counting and hemoglobin determination. The reagent prevents platelet aggregation and improves the accuracy of the white blood cell count.

Reagent for combined diluting and lysing whole blood

A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.

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Miami Lakes, FL, United States of America

Fred L Larsen