The Salk Institute for Biological Studies

University of California

Other

The Scripps Research Institute

Mars, Incorporated

Arch Development Corporation

Signal Pharmaceuticals, Inc.

Sidney Kimmel Cancer Center

San Diego Regional Cancer Center

Fred Harrison Gage

Jasodhara Ray

Theodore Friedmann

Steven T Suhr

Brian K Kaspar

Michael B Rosenberg

Tomoko Kuwabara

David Barba

Peter G Schultz

Jon Asher Wolff

Sheng Ding

Mark Henry Tuszynski

Robert E Sobol

Ivor Royston

Alysson Renato Muotri

Malcolm Schinstine

Michael D Kawaja

Masaki Warashina

Kyung Hoon Min

Theo Palmer

Maria C N Marchetto

Jun Takahashi

Roger C Guillemin

Daniel A Peterson

Jeffrey D Rothstein

Habib Fakhrai

Mark Allen Kelm

Dinah W Y Sah

Un Jung Kang

John F Hammerstone

James Bradley Aimone

Tetsuya Ueba

Kazuhiro Sakurada

Rav Grill

Janet Wiles

Theodore Friedman

Minoru Hoshimaru

Elad B Gil

Marie-Claude C Senut

Francis G Safar

Masayo Takahashi

Philippe J Taupin

Henriette Van Praag

Cedric Bardy

A method for extracting stem cells from a non-embryonic stem cell source, including providing a non-embryonic stem cell source including stem cells; perfusing the non-embryonic stem cell source with a pulsatile flow of a perfusion solution to produce a perfusate including stem cells and a perfused non-embryonic stem cell source; and isolating the stem cells from the perfusate to produce isolated stem cells, is provided. Also provided is a non-embryonic stem cell line derived from a non-embryonic stem cell obtained using the pulsatile perfusion extraction method.

Pulsatile perfusion extraction method for non-embryonic pluripotent stem cells

Provided herein are methods and compositions useful in treating neurological disorders.

Method for preparing an X chromosome inactivated female human neural cell

Methods and compositions for the generation and use of footprint-free human induced pluripotent stem cells are provided.

Induced pluripotent stem cell produced by transfecting a human neural stem cell with an episomal vector encoding the Oct4 and Nanog proteins

An apparatus, article and method containing an artificial neural network that, after training, produces new trainable nodes such that input data representative of a first event and input data representative of a second event both activate a subset of the new trainable nodes. The artificial neural network can generate an output that is influenced by the input data of both events. In various embodiments, the new trainable nodes are sequentially produced and show decreasing trainability over time such that, at a particular point in time, newer produced nodes are more trainable than earlier produced nodes. The artificial neural network can be included in various embodiments of methods, apparatus and articles for use in predicting or profiling events.

Temporally dynamic artificial neural networks

The present invention provides compositions and methods for inducing neuronal cell differentiation.

Compositions and methods for inducing neuronal differentiation

The invention provides a method for modulating gene expression by contacting a cellular system with a double-stranded ribonucleic acid molecule capable of associating with a regulatory machinery that controls transcription of one or more genes, wherein the association results in altered expression of the one or more genes. The invention is further directed to method for directing the differentiation of neuronal stem cells into neurons by contacting a cellular system with a double-stranded ribonucleic acid molecule capable of associating with a regulatory machinery that controls transcription of one or more genes involved in neuronal differentiation and directing the transcription of the one or more genes. In related embodiments, the invention provides particular compositions of double-stranded ribonucleic acid molecules as well as therapeutic and screening applications of the invention.

Transcriptional regulation of gene expression by small double-stranded modulatory RNA

The invention provides a method for improving cognitive performance in a physically active subject. The invention further provides a method of enhancing neurogenesis in a physically active subject. In one embodiment, the method encompasses administering to the subject an effective amount of one or more flavonoids. In a further embodiment, the method encompasses administering to the subject an effective amount of one or more antioxidants.

Method for increasing cognitive function and neurogenesis

A method for producing a neuroblast and a cellular composition comprising an enriched population of neuroblast cells is provided. Also disclosed are methods for identifying compositions which affect neuroblasts and for treating a subject with a neuronal disorder, and a culture system for the production and maintenance of neuroblasts.

Method for production of neuroblasts

Methods are disclosed for delivering a heterologous gene to a cell body of a neuron by contacting a muscle tissue innervated by the neuron with a viral vector comprising a heterologous gene, wherein the viral vector enters said neuron and is retrogradely moved to the cell body. Additionally, methods for expressing secreted proteins from a nerve cell body as well as methods for treating neurodegenerative disorders such as amyotrophic lateral sclerosis are described.

Targeted retrograde gene delivery to motor neurons

The invention provides chimeric proteins having at least two functional protein units, each containing the dimerization domain of a member of the steroid/thyroid hormone nuclear receptor superfamily. The chimeric proteins can fold under crystallization conditions to form functional entities. The functional entities optionally contain a novel flexible peptide linker of variable lengths between at least two of the protein units. In a preferred embodiment, the linker is designed to be increased in increments of 12 amino acids each to aid in preparation of variant chimeric proteins. The DNA binding characteristics of the invention functional entities differ from those of wild-type complexes formed between 'monomeric' receptors and their binding partners. Some functional entities, e.g. dimers expressed as fusion proteins, transactivate responsive promoters in a manner similar to wild-type complexes, while others do not promote transactivation and function instead essentially as constitutive repressors. The invention further provides nucleotide sequences encoding the invention chimeric proteins, cells containing such nucleotide sequences, and methods for using the invention chimeric proteins to modulate expression of one or more exogenous genes in a subject organism. In addition, isolated protein crystals suitable for x-ray diffraction analysis and methods for obtaining putative ligands for the invention chimeric proteins are provided.

Hormone receptor functional dimers and methods of their use

Methods are disclosed for transducing neurons with heterologous genes using retrograde viral transport. The methods disclosed employ substantially non-toxic vectors, such as adeno-associated virus vectors, that are capable of retrograde axonal transport to introduce and express genes in the neurons. This method has applications in the mapping of neural pathways, in stimulating or inhibiting the growth of neurons, and in the treatment of various neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis.

Targeted retrograde gene delivery for neuronal protection

In accordance with the present invention, it has been discovered that nuclear receptor proteins isolated from the silk moth(bR) are useful for the regulation of transgene expression. bR is generally thought to be a strong transcriptional regulator within cells of the silk moth. In accordance with the present invention, it has been discovered that bR is also functional in mammalian cells. It has further been discovered that the addition of activation domains to the bR open-reading frame enhances the activity of the ligand modulated regulator to afford high-level transcriptional induction. Further modifications to the bR ligand binding domain result in receptors with unique transactivational characteristics.

Modified lepidopteran receptors and hybrid multifunctional proteins for use in transcription and regulation of transgene expression

The present invention is directed to methods of repairing damaged or diseased, specialized or differentiated tissue in mature animals, particularly neuronal tissue such as retinas. In particular, the invention relates to transplantation of adult, hippocampus-derived progenitor cells into a selected neural tissue site of a recipient. These cells can functionally integrate into mature and immature neural tissue. The invention encompasses, in one aspect, repopulating a retina of a dystrophic animal with neurons, by injecting clonally derived, adult central nervous system derived stem cells (ACSC) derived from a healthy donor animal into an eye of the dystrophic recipient. Herein disclosed is the first successful and stable integration of clonally derived ACSC into same-species but different strain recipients (e. g., Fischer rat-derived adult hippocampal derived progenitor cells (AHPCs) into dystrophic RCS rats). Surprisingly, AHPCs were also found to integrate successfully into a xenogeneic recipient (e.g., rat AHPCs into the retina of dystropic rd-I mice).

Method of isolating adult mammalian CNS-derived progenitor stem cells using density gradient centrifugation

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La Jolla, CA, United States of America

Fred Harrison Gage