Bio-rad Pasteur

Bio-rad Innovations

Centre National De La Recherche Scientifique

Universitè De Montpellier

François Yves Rieunier

Nadine Lambert

Muriel Feyssaguet

Stéphanie Henriot

Isabelle Karine Giuliani

Bernard Pau

Jean-Francois Hubert Delagneau

Stephane Jean Xavier Gadelle

Fabienne Babin

Catherine Christiane Marie Larue

Denis Marie Bernárd Chenebaux

Sylvie Marie-France Trinquier

Isabelle Nathalie Garric

Laurence Hamon

The invention relates to a method for detecting, in vitro, an infection with a microorganism, such as the hepatitis C virus, in a biological sample, by simultaneously detecting an antigen of this microorganism and the antibodies against this same antigen, and also to the reagents and kits implementing this method.

Method for simultaneously detecting an antigen of, and an antibody against, an infectious microorganism

Method for simultaneously detecting an antigen of, and an antibody, against, an infectious microorganism

The invention concerns the use of at least a synthetic compound comprising: a carrier molecule having at least a hydrocarbon chain comprising at least 10, advantageously at least 12, preferably at least 14 and not more than 300, advantageously not more than 100, preferably not more than 50, selected'among C, N, O, S, P and Si, and comprising at least two reactive functions, or capable of reacting, for grafting lateral arms on said chain, and at least two different epitopes carried by said lateral arms grafted on said carrier molecule, as standard or control for immunoassay of said biological molecules, in particular of polypeptides or proteins.

Use of stabilised synthetic compounds in immunoassay

The invention relates to an in vitro diagnosis of heart failure. More specifically, the invention relates to specific antibodies of a peptide domain which is situated on either side of hinge region R76S77 of proBNP(1-108). In particular, the invention relates to a method of obtaining the aforementioned antibodies and to the use thereof in detecting blood proBNP(1-108) with the exception of BNP(1-76) and BNP(77-108). The invention also relates to a method of detecting blood proBNP(1-108), reagents and a kit for same.

Specific antibodies for diagnosing heart failure

The invention relates to a method of immobilizing an affinity reagent or affinity reagents on a hydrophobic solid phase which can be used in biological assays for analyte detection. The invention relates more particularly to a method of immobilizing an affinity reagent on a hydrophobic solid phase functionalized by a carboxyl group, said method comprising a step for activation of said solid phase and a step for coupling of the affinity reagent to said solid phase, characterized in that the step for activation of said solid phase uses a combination of a carbodiimide and a phosphate buffer in the presence of a co-activator and in an acid medium, and in that the coupling step is performed in a basic medium. The invention further relates to the reactive complexes obtained by this method and to their use in immunoassay kits, hybridization kits or enzymatic assay kits.

Method for immobilization of (an) affinity reagent(s) on a hydrophobic solid phase

Synthetic peptides of the monomer type with 13 to 33 amino acids, in linear form or in a form cyclized by means of inter-cysteine disulphide bridges, have the general formula (I):&#x394;-Z-TrpGlyCys-&#x398;-CysTyrThrSer-&#x3a9;&#x2003;&#x2003;(I)wherein &#x394; is a biotinyl radical, a biocytinyl radical, a hydrogen atom, an acetyl (CHCO&#x2014;) radical, an aliphatic chain which may contain one or two thiol, an aldehyde functional group, or an amine functional group, Z represents peptide sequence -&#x39e;-Ser-&#x39e;-, -&#x39e;-Gln-&#x39e;-, or -&#x39e;-Asn-&#x39e;-, wherein -&#x39e;represents a peptide sequence of 0 to 9 amino acids and -&#x39e;represents a peptide sequence of 0 to 5 amino acids, &#x398; is -Arg Gly Arg Leu Ile-(SEQ ID NO: 15), -Arg Gly Arg Leu Val-(SEQ ID NO: 16), -Arg Gly Lys Leu Ile- (SEQ ID NO: 17), -Arg Gly Lys Leu Val-(SEQ ID NO: 18), -Lys Gly Arg Leu Ile-(SEQ ID NO: 19), or -Lys Gly Arg Leu Val-(SEQ ID NO: 20), &#x3a9;, attached to the &#x2014;CO&#x2014; group of serine, is a hydroxyl (&#x2014;OH) radical, an amino (&#x2014;NH) radical, an alkoxy radical having 1 to 6 carbon atoms, a peptide sequence of formula Val-&#x3a3;-&#x3a8; wherein &#x3a3; represents a sequence of formula -(AA)-Trp Asn-(AA)-(AA) wherein (AA) represents an amino acid different from lysine, (AA) represents an amino acid, and (AA) is serine or a threonine residue, and &#x3a8;, attached to the &#x2014;CO&#x2014; residue of the free AAamino acid, is OH, NH, or an alkoxy radical having from 1 to 6 carbon atoms, and a peptide sequence of formula -Val-&#x3a8; wherein &#x3a8;, attached to the &#x2014;CO&#x2014; residue of valine, is OH, NH, or an alkoxy radical having from 1 to 6 carbon atoms.

Synthetic peptides useful in biological assays for detecting infections caused by group O HIV-1 viruses

Growing community of inventors

Fontenay le Fleury, France

François Yves Rieunier