Biochemie Gesellschaft M.b.h

Sandoz Ag

Agrosolution Gmbh&co. Kg

Franz Knauseder

Norbert Palma

Jörg Windisch

Günter Stempfer

Peter Huemer-Hartl

Kurt Schoergendorfer

Hans Boehling

Tillmann Rumenapf

Heinz J Thiel

Heinz-Jürgen Thiel

Waander Riethorst

Arno Reichert

Kurt Sch&ouml;rgendorfer

Martin Schiestl

Tillmann Rümenapf

The invention relates to a novel aqueous suspension preparation comprising a water-suspended microparticulate calcite mineral with a particle diameter &#x2264;35 &#x3bc;m, plant extract, and a surfactant that can shift the zeta potential of the water-suspended microparticulate calcite mineral into the negative range, and the use of this aqueous suspension preparation as a foliar fertilizer. The invention also relates to a foliar fertilizer comprising this aqueous suspension preparation.

Aqueous suspension preparations and the use of same as leaf fertilizers

The present invention relates to an expression vector, comprising a polynucleotide encoding a fusion protein comprising the signal sequence of the gac gene ofand a polypeptide of interest, a prokaryotic host cell transformed with such an expression vector and a process for production of a polypeptide of interest using said host cell and said expression vector.

Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides

The present invention relates to a process for the production of a heterologous polypeptide with homogeneous N-terminus in a bacterial host cell, wherein the heterologous polypeptide is autoproteolytically cleaved from an expressed fusion protein which comprises a polypeptide with the autoproteolytic activity of an autoprotease Nof a pestivirus and the heterologous polypeptide by the Nautoproteolytic activity.

Production of proteins by autoproteolytic cleavage

The present invention relates to a process for the recombinant production of a desired heterologous polypeptide with a clearly defined homogenous N-terminus in a bacteria host cell. A fusion comprising a peptide with the autoproteolytic activity of an autoprotease Nof a pestivirus and the heterologous polypeptide is initially expressed in the form of cytoplasmic inclusion bodies in the host cell, the inclusion bodies are isolated and subsequently treated so that the desired heterologous polypeptide is cleaved autoproteolytically by the Nactivity of the fusion protein.

Production of heterologous proteins using an Npro autoprotease of a pestivirus and inclusion bodies

The present invention relates to a nucleic acid molecule which codes for the cephalosporin acetylesterase from ATCC 6633 (DSM 11909), vectors and host cells which comprise such a nucleic acid molecule, a process for the recombinant preparation of cephalosporin acetylesterase from ATCC 6633 (DSM 1 1909) using said nucleic acid molecule, and a process for preparing 3-deacetylcephalosporin compounds.

Nucleic acid molecule encoding a cephalosporin acetylesterase

A process is presented for producing spherical particles containing microorganism cells having desired enzyme activity. The process comprises the steps of mixing the cells directly with a primary or secondary amine-containing polymer, combining the resulting mixture with an organic solvent to form a two-phase system, and then adding a bifunctional cross-linking agent to yield the spherical particles. The preferred enzyme activities are D-amino acid oxidase and glutarylacylase activities.

Spherical particles containing microorganism cells having enzyme activity

Growing community of inventors

Kirchbichl, Austria

Franz Knauseder