Smithkline-rit

Smithkline Biologicals

Frans V Wijnendaele

Guy Simonet

Daniel Gilles

The process is applicable to the supernatant of engineered yeast cells disrupted in the presence of a non-ionic detergent; it comprises the precipitation of contaminants by polyethylene glycol and the treatment of this latter supernatant with either a bivalent metal cation or, after eventual ultrafiltration, with ammonium sulfate.

Process for the extraction and purification of proteins from culture

E. coli heat stable (ST) enterotoxin derivatives are prepared by reaction of E. coli ST enterotoxin with crosslinking agents for proteins. The so-obtained crosslinked ST enterotoxins show immunogenic activity; they are valuable prophylactic agent for protecting man and animals against diarrheal disease provoked by E. coli enterotoxin and they may be formulated into compositions for intramuscular, subcutaneous or oral administration.

Growing community of inventors

Ottenburg, Belgium

Frans V Wijnendaele