Mayo Foundation for Medical Education and Research

Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Franklin R Cockerill, Iii

James R Uhl

Lynne M Sloan

Astrid Reiser

Christian Aichinger

Bruce C Kline

Thomas F Smith

Robin Patel

Sabine Lohmann

Ulrike Salat

Jon E Rosenblatt

Randall T Hayden

The present invention relates to methods of detecting the presence or absence of methicillin-resistant(MRSA) in a sample, the method comprising performing an amplifying step, a hybridizing step and a detecting step. Furthermore, the present invention relates to primers, probes and kits for the detection of MRSA.

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The invention provides methods to detectand/orin a biological sample. Primers and probes for the differential detection ofandare provided by the invention. Articles of manufacture containing such primers and probes for detectingand/orare further provided by the invention.

The invention provides methods to detectin biological samples using real-time PCR. Primers and probes for the detection ofare provided by the invention. Articles of manufacture containing such primers and probes for detectingare further provided by the invention.

Detection of clostridium difficile

The invention provides methods to detect group B(GBS) in biological samples using real-time PCR. Primers and probes for the detection of GBS are provided by the invention. Articles of manufacture containing such primers and probes for detecting GBS are further provided by the invention.

Detection of bordetella

The invention provides methods to detect group B streptococcus (GBS) in biological samples using real-time PCR. Primers and probes for the detection of GBS are provided by the invention. Articles of manufacture containing such primers and probes for detecting GBS are further provided by the invention.

Detection of group B streptococcus

The invention provides methods to detect vancomycin-resistantin biological samples using real-time PCR. Primers and probes for the detection of vancomycin-resistantare provided by the invention. Articles of manufacture containing such primers and probes for detecting vancomycin-resistantare further provided by the invention.

Detection of vancomycin-resistant enterococcus spp.

The invention provides methods to detect mecA-containingspp. in biological samples using real-time PCR. Primers and probes for the detection ofare provided by the invention. Articles of manufacture containing such primers and probes for detecting mecA-containingspp. are further provided by the invention.

The invention provides methods to detect and further to detect in biological samples using real-time PCR. Primers and probes for the detection of and are provided by the invention. Articles of manufacture containing such primers and probes for detecting and are further provided by the invention.

Detection of Legionella

The invention provides methods to detect Group A Streptococcus (GAS) in biological samples using real-time PCR. Primers and probes for the detection of GAS are provided by the invention. Articles of manufacture containing such primers and probes for detecting GAS are further provided by the invention.

Detection of group a Streptococcus

A method for selectively detecting M. tuberculosis is provided employing restriction fragment length polymorphism analysis of an enzymatic digest of the M. tuberculosis katG gene.

Mutations in the katG gene useful for detection of M. tuberculosis

A method for determining the susceptibility of a strain of M. tuberculosis to isoniazid is provided comprising employing the techniques of restriction length polymorphism analysis to determine whether or not the DNA of said strain has an NciI-MspI restriction site at the codon corresponding to codon 463 of an M. tuberculosis katG gene consensus sequence.

Detection of isoniazid resistent strains of M. tuberculosis

A method for determining the susceptibility of a strain of M. tuberculosis to isoniazid is provided comprising employing the techniques of restriction fragment length polymorphism analysis to determine whether or not the DNA of said strain has an MspI restriction site at the codon corresponding to codons 315 or 463 of an M. tuberculosis katG gene consensus sequence.

Growing community of inventors

Rochester, MN, United States of America

Franklin R Cockerill, Iii