Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Gsf-forschungszentrum Fur Umwelt Und Gesundheit, Gmbh

Frankgen Biotechnologie Ag

Gie-cerbn, Centre Europeen De Recherche En Biologie Et En Medecine (gie)

Helmholtz Zentrum Munchen Deutsches Forschungszentrum Fur Gesundheit Und Umwelt

Frank Schnütgen

Pierre H Chambon

Wolfgang Wurst

Norbert B Ghyselinck

Harald Von Melchner

Patricia Ruiz

Jens Hansen

Silke De-Zolt

Thomas Floss

The present invention relates to a novel class of gene trap vector (enhanced gene trap vectors, eGTV) for efficiently identifying silent or weakly expressed target genes in mammalian genomes, methods of their production and methods for identifying and mutating target genes by using the enhanced gene trap vectors. The gene trap vectors of the present invention can also be used for inducing the expression of silent genes and enhancing the expression of weakly expressed genes. The use of the enhanced gene trap vectors for creating transgenic organisms to identify gene function and to validate pharmaceutical compounds prior to clinical applications is a further aspect of the present invention.

Enhancer-containing gene trap vectors for random and targeted gene trapping

The invention relates to a method for the stable inversion of a DNA fragment upon recombinase-mediated rearrangements using two sets of two incompatible site-specific recombinase targeting sites (SSRTS) in the same order but in reverse orientation flanking the DNA fragment to be inverted. The invention also relates to a method for the stable inversion of the DNA fragment upon rearrangement mediated by a recombinase such as Cre recombinase. The invention also relates to a method for obtaining a transgenic cell of which at least one allele of a DNA sequence of interest is invalidated by a process of conditional deletion and the genome of which has a reporter gene inserted at the place of the DNA fragment deleted by the process of conditional deletion. A method to generate targeting sites to perform site-specific recombination mediated cassette exchange is also provided.

Method for the stable inversion of DNA sequence by site-specific recombination and DNA vectors and transgenic cells thereof

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Alzenau, Germany

Frank Schnütgen