Pieris Ag

Hoechst Schering Agrevo Gmbh

Aventis Cropscience Gmbh

Frank Schmidt

Gerald Beste

Thomas Stibora

Arne Skerra

Wolfgang R Streber

Bernhard Grimm

Arne Skerre

Susanna Gr&auml;fe

Frank H&auml;nel

Jutta Papenbrock

G&uuml;nter Donn

The invention relates to the production of novel proteins exhibiting binding activity for certain ligands, the so-called anticalins. To this end, the structure of peptides of the lipocalin family is modified by amino acid replacement in their natural ligand binding pocket using genetic engineering methods. Like immunoglobulin, the anticalin thus obtained can be used to identify or bind molecular structures.

Anticalins

The invention relates to the production of novel proteins exhibiting bonding activity for certain ligands, the so-called anticalins. To this end, the structure of peptides of the lipocalin family is modified by amino acid replacement in their natural ligand binding pocket using generic engineering methods. Alike immunoglobulin, the anticalin thus obtained can be used to identify or bond molecular structures.

The invention relates to the production of novel proteins exhibiting bonding activity for certain ligands, the so-called anticalins. To this end, the structure of peptides of the lipocalin family is modified by amino acid replacement in their natural ligand binding pocket using genetic engineering methods. A like immunoglobulin, the anticalin thus obtained can be used to identify or bond molecular structures.

The present invention relates to a nucleic acid molecule which encodes a protein with the function of a plant Mg chelatase subunit CHLD or an active fragment thereof; a protein which has the function of a plant Mg chelatase subunit CHLD or an active fragment thereof, preferably a recombinant protein; a method of determining the interaction of plant Mg chelatase subunits, in which a host cell is transformed with a DNA sequence as claimed in one or more of claims 1 to 3 and at least with one DNA sequence encoding a further subunit of Mg chelatase in such a manner that the interaction of the Mg chelatase gene products leads to a directly or indirectly, qualitatively or quantitatively measurable signal, preferably by activating a marker gene, and transgenic plants, transgenic plant cells, transgenic plant organs, transgenic plant seeds, transgenic propagation material comprising an abovementioned nucleic acid molecule.

DNA sequences encoding for subunit CHLD of plant magnesium chelatases and determining the activity of plant magnesium chelatases

Provided is a method for producing transgenic monocotyledonous plants, plant cells, plant parts, seeds, and reproduction material with modified 5-aminolevulinic acid biosynthesis. This is achieved by stably integrating one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS) isolated from the alpha group of purple bacteria, an active fragment thereof or an antisense or complementary sequence thereof, into tie plant genome in stable form. This method can also be used to control undesired vegetation Also provided is a method for producing transgenic plants or plant cells whose glutamate-1-semialdehyde transferase (GSAAT) expression is suppressed or inhibited by stable integration of at least one nucleic acid molecule encoding an ALAS isolated from the alpha group of purple bacteria into the plant plastome by plastid transformation.

Method for producing transgenic plants with modified 5-aminolevulinic acid biosynthesis, method for identifying 5-aminolevulinic acid synthesis effectors

Growing community of inventors

Frankfurt-am-Main, Germany

Frank Schmidt