Geneart Ag

Life Technologies Corporation

Life Technologies As

Thermo Fisher Scientific Geneart Gmbh

Frank Notka

Geir Fonnum

Kevin G Hoff

Ralf Wagner

Marcus Graf

David Raab

Xiquan Liang

Thomas Poehmerer

Axel Trefzer

Korbinian Heil

Kristian Andersson

Federico Katzen

Daniela Daubert

Claudia Benkel

Phillip Kuhn

Doris Leikam

Andreas Zeidler

Diana Hammer

Doris Baumann

Michael Liss

Matthias Arnold

Jutta Derer

Ansgar Romig

Stephen Whiteside

Jonathan Chesnut

The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

High efficiency, small volume nucleic acid synthesis

The invention relates to nucleic acid modifications for a directed expression modulation by the targeted insertion or removal of CpG dinucleotides. The invention also relates to modified nucleic acids and expression vectors.

Method for modulating gene expression by modifying the CpG content

The present invention relates to a method for identifying frameshift mutations in coding nucleic acid sequences.

Method for determining frameshift mutations in coding nucleic acids

The invention relates to vector constructs for an HIV-specific gene therapy. The expression of transgenes is coupled with an infection of the cell with HIV while the transcription of the transgene is controlled by a transcription control region derived from HIV. In addition, the transgene is improved with regard to RNA stability and expression efficiency by modifying the nucleotide sequence.

Inducible gene expression

The invention relates to a method for optimizing a nucleotide sequence for expression of a protein on the basis of the amino acid sequence of the protein, in which for a particular region there is specification of a test sequence with m optimization positions on which the codon occupation is varied, a quality function being used to ascertain the optimal codon occupation on these optimization positions, and one or more codons of this optimal occupation being specified as codons of the optimized nucleotide sequence. These steps are iterated, with the codons of the optimized nucleotide sequence which are specified in the preceding steps remaining unchanged in subsequent iteration steps. The invention additionally relates to a device for carrying out this method.

Method and device for optimizing a nucleotide sequence for the purpose of expression of a protein

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Wuppertal, Germany

Frank Notka