Temple University

Proplex Technologies, LLC

Cedars-sinai Medical Center

Frank N Chang

George P Tuszynski

Michael J Gehret

Phu T Duong

Christopher R Yonan

Tully J Speaker

Leland W K Chung

George Paul Tuszynski

Yi Zhang

Ruoxiang Wang

Stefan Mrdenovic

Stephen C Hsu

Solomon Luo

Jeff Yang

Gina Chia-Yi Chu

A method for detecting cells having granules expressing G protein coupled receptor-associated sorting protein 1 (GASP-1) or a fragment thereof is provided. The detection method may comprise: (a) contacting the cells with an effective amount of a binding protein, wherein the binding protein comprises an antigen binding fragment that specifically binds GASP-1; and (b) identifying cells having granules bound to the binding protein. The GASP-1 granules may be in the cytosol or on the surface of the cells. Also provided are methods for producing T-cells comprising a chimeric antigen receptor, anti-GASP-1 antibody or a bi-specific binding protein. Further provided are methods for treating GASP-1-mediated to disease or inactivating exosomes, microvesicles or oncosomes.

Binding proteins and chimeric antigen receptor T cells targeting GASP-1 granules and uses thereof

An embodiment of the invention relates to the use of stabilized cancer peptide fragments derived from 'redoxin proteins' selected from the group consisting of thioredoxin; peroxiredoxin-1, 2 and 3; glutaredoxin-3; glutathione peroxidase-4; and nucleoredoxins for the diagnosis of cancers, particularly pancreatic cancer. A method for the detection of cancer, severity of cancer, and/or effectiveness of a therapeutic regimen comprises detecting and/or measuring the amount of redoxin peptide fragments present in the biological sample of a subject.

Stabilized peptide fragments from nucleoredoxin X1 and uses thereof

Methods for identifying disease-specific markers, in particular breast cancer markers, by electrophoretically separating serum albumin complexes in a biological sample on a membrane are provided. Electrophoretic separation profiles representing different diseases or different cancer stages can be produced, and used in the diagnosis, prognosis and treatment of these diseases. Methods for identification of a cancer peptide fragment comprising a cancer peptide motif are provided. Also provided are breast cancer and other cancer markers and antibodies that specifically recognize these markers.

Serum markers associated with early and other stages of breast cancer

A method for determining whether early stage cancer is present in a subject comprises detecting the expression level of GASP-1 in the subject by detecting the amount of GASP-1 peptide fragments present in a biological sample of the subject. Because cancer can be detected at an early stage, therapeutic targeting may be initiated before cancer reaches late stage (e.g., before the development of overt symptoms). A method for treating early stage cancer in a subject comprises administering to the subject an effective amount of a GASP-1 inhibitor to inhibit the progression of early stage cancer to late stage cancer. A Competitive ELISA capable of detecting GASP-1 peptide fragments at a concentration of less than 1 ng/ml was developed.

G-protein coupled receptor-associated sorting protein 1 as a cancer biomarker

Disease specific markers, in particular cancer markers, can be detected by electrophoretically separating proteins and protein complexes from a biological sample on a protein binding polymeric membrane in a low conductivity, water-miscible organic solvent buffer. Electrophoretic separation profiles representing different diseases can be produced, and used in the diagnosis or prognosis of these diseases.

Method for detecting disease markers

A method of visualizing proteins bound to a protein-binding membrane is provided herein, comprising reacting proteins bound to protein-binding membranes with a compound of formula (I) wherein R, R, R, R, R, M, n, and m are as defined herein. 

Method of visualizing proteins bound to protein-binding membranes

Proteins can be rapidly separated to a high degree of resolution by electrophoresis on polymeric membranes that have high protein binding capacity. The electrophoretic separation is carried out in a low conductivity, water-miscible organic solvent buffer. The low conductivity of the organic solvent buffer minimizes heat generation, and the water-miscible nature of the organic solvent buffer permits the analysis of hydrophobic and low molecular weight proteins as well as hydrophilic proteins. When electrophoresis is conducted under non-denaturing conditions, it allows the detection of enzymatic activities, protein-protein interactions and protein-ligand interactions.

System and methods for electrophoretic separation of proteins on protein binding membranes

The present invention is directed to a method and a test kit for detecting cockroach allergens and/or determining the total allergen level. A sample from an environment in which cockroaches are present or are suspected to be present is provided. The sample and a substrate composition comprising a chitinase substrate are then contacted. The presence of cockroach allergens can be determined by observing or detecting the magnitude of a measurable change of a property of the substrate composition following the contact between the sample and the substrate composition wherein the magnitude of the measurable change of a property of the substrate composition is proportional to the total allergen level. The total allergen level can be determined by measuring the magnitude of the measurable change of a property of the substrate composition, or by comparing the magnitude with a reference standard.

Method for detecting cockroach allergens and determining total allergen level

An insecticidal product comprises a solid substrate impregnated with sterile cockroach aggregation pheromones, a sterile insecticidal composition comprises a solvent extract of the product, which may be in solution, and an insect attractant product comprises a solid substrate having the composition partially or fully coated thereon. The product and composition of the invention may be utilized in the manufacture of insect traps provided with a hollow receptacle and an opening, and optionally, a cover. An insect trap may comprise a hollow receptacle with a slippery internal surface to prevent the exit of the insects, and an opening, and optionally a cover. The present products are particularly suitable for trapping insects, such as cockroaches.

Compositions containing cockroach aggregation pheromones, their

An insecticidal composition comprising a first composition having an effective amount of at least one species of entomopathogen distributed in a matrix. A second composition selected from vegetable oil, crop oil and partially hydrogenated oil containing mono- and di-glycerides surrounds the first composition and significantly prolongs nematode viability. The matrix is a nematode-containing macrogel or paste of partially hydrogenated oils containing mono- and di-glycerides. The macrogel matrix is selected from a continuous polymer macrogel or microcapsule-containing macrogel. Partially hydrogenated oils containing mono- and di-glycerides, (e.g., Crisco.RTM. shortening), can also be mixed with free nematodes to produce a paste formulation. Trapping nematodes in a Crisco.RTM. matrix formulation along with a water retentive polymer results in increased protection against desiccation and significantly prolongs viability during storage. An insect feeding stimulant, such as raffinose, may also be added to the mix.

Stabilized insect nematode compositions

An insect bait station comprising a first compartment with a hydrated macel containing at least one species of entomopathogen and a second compartment containing a hydrated water retentive compound layer which acts as a water-reservoir for the entomopathogen.

Insect bait station

An insecticidal composition in the form of a hydrated macrogel containing at least one species of entomopathogen and a hydrated water retentive compound which acts as a water-reservoir for the entomopathogen. Optionally, the macrogel contains attractants, in particular raffinose and gamma-irradiated, fungal-decayed wood.

Insecticide delivery system and attractant

Delivery system for topical application comprising a highly viscous carrier containing dissolved or dispersed and microencapsulated topically-active agents providing immediate and sustained release of the topically-active agent to localized body sites.

Growing community of inventors

Dresher, PA, United States of America

Frank N Chang