Tecan Trading Ag

Frank Münchow

Andreas Erlbacher

Lutz Niggl

Markus Schürf

Alexander Kaser

Christian Oberdanner

An imaging system and method are provided in which a well of a microplateis imaged by a cameracomprising magnification optics. The camera is controlled to acquire a series of images of the well with different exposures. The series of images comprise a base image with a base exposure and at least one further image with a larger exposure than the base exposure. The series of images are then merged into an output image which comprises in a center region of the well image content from the base image and at a peripheral region of the well image content from the at least one further image. Advantageously, the output image may allow for better assaying or analysis of the samples in the well than any of the individual images.

Imaging a well of a microplate

A spectrometer () comprises a light source (), a monochromator () with at least one diffraction grating (), a monochromator housing (), an order sorting filter (), a microplate receptacle () and a controller (). The order sorting filter () of the spectrometer () comprises a substrate (), a first optical thin film () and a second optical thin film (), wherein, in a spatially partly overlapping and interference-free manner, the first optical thin film () is arranged on a first surface () and the second optical thin film () is arranged on a second surface () of the substrate (). A spectrometer () equipped with a respective order sorting filter is used in a scanning method for detecting the absorption spectrum of samples examined in wells () of microplates ().

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Munich, Germany

Frank Münchow