Bayer Cropscience Nv

Basf Se Corporation

Bayer Bioscience N.v.

Commonwealth Scientific and Industrial Research Organisation

Basf Agricultural Solutions Seed US LLC

Plant Genetic Systems

Other

Bayer Cropscience Ag

Bayer Cropscience Gmbh

Texas Tech University System

The University of Edinburgh

Guerriero, Gea

Bulone, Vincent

Frank Meulewaeter

Marc De Block

Gerben Van Eldik

Rainhard Koch

Veronique Gossele

Bernd Essigmann

Stefan K Jansens

Michael Metzlaff

Roel Van Aarssen

Piet Soetaert

Danny James Llewellyn

Stephen C Fry

Greet Vanderkimpen

Marcus Cornelissen

Christophe Liseron-Monfils

Andrew Hudson

Claire Holland

Tom J Simmons

Lenka Frankova

Kyle E Mohler

Ilse Van Den Brande

Jan Dittgen

Udo Matthias Bickers

Jeroen Van Rie

Kathleen D'Halluin

Ruediger Hain

Linda Trolinder

John Jacobs

Thomas Wilde

Marc Cornelissen

Hal Moser

Bartel Vanholme

Shirong Zhang

Vincent Bulone

Gengxiang Jia

Ina C A Faché

Veronique M L Gosselé

Marie-Therese Scheirlinck

Gea Guerriero

Gary Henniger

Koen Weterings

Forrest Sheng Bao

Arnab Ghosh

Zhixin Xie

Bernadette Saey

Kurt Boudonck

Martine Vandewiele

The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.

Regulatory nucleic acid molecules for enhancing gene expression in plants

The present invention relates to novel gene sequences encoding insecticidal proteins produced bystrains. Particularly, new chimeric genes encoding a CryIC, CryIB or CryID protein are provided which are useful to protect plants from insect damage. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising one of such chimeric gene and at least one other such chimeric genes.

Genes encoding insecticidal proteins

The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.

Hetero-transglycosylase and uses thereof

The present application discloses a recombinant fiber-selective promoter region comprising a DNA molecule comprising a fiber specificity region of a cotton lipid transfer protein gene promoter, operably linked to a DNA molecule comprising a nucleotide sequence having at least 90% sequence identity to a nucleotide sequence of about 500 consecutive nucleotides of the 3' end of the FB8-like 2 promoter and use thereof to increase fiber-selective expression of products of interest in cotton fiber cells.

Recombinant promoter with increased fiber-specific expression

Methods and means are provided to produce positively charged oligosaccharides in the plant cell wall by introducing into said plant cell a Nodulation C protein fused to a heterologous Golgi signal anchor sequence.

Methods for altering the reactivity of plant cell walls

An isolated nucleic acid molecule is provided comprising a nucleotide sequence which encodes a glutamine:fructose-6-phosphate amidotransferase fromwhich is particularly suitable for expression in cotton plant cells. The invention also relates to plant cells or plants, in particular to cotton plant cells or cotton plants which produce an increased amount of positively charged polysaccharides in their cell walls. Furthermore methods and means are provided to increase the content of positively charged polysaccharides in the cell walls of cotton cells, in particular in cotton fibers. Fibers obtained from such cotton plants have an altered chemical reactivity which can be used to attach reactive dyes or other textile finish reagents to these fibers.

Cotton fibers with increased glucosamine content

The present invention relates to novel DNA sequences encoding insecticidal Cry1 C proteins derived from, and their use in plants to control insect pests. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising a Cry1 C chimeric gene of the invention and at least one other chimeric gene, such as a chimeric gene encoding an insecticidal Cry1Ab protein, and methods of making or using such plant cells or plants.

Methods and means are provided to enhance the selective expression of transgenes under control of a fiber-selective promoter, in fiber cells, particularly cotton fiber cells by including target sites for naturally occurring microRNAs with a specific expression profile, particularly with a differential expression profile between cells leading to fibers and other cells of the fiber producing plant, into the transcribed region of genes of interest.

Enhanced selective expression of transgenes in fiber producing plants

Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1&#xd7; of at least one HPPD inhibitor, and methods for making such plants.

Methods and means to modify a plant genome

Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

Cotton fibers having positively charged oligosaccharides

Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding achitin synthase in cotton plants.

Methods for manufacturing plant cell walls comprising chitin

The present invention relates to materials and methods for the expression of a gene of interest selectively in cotton fibers. In particular, the invention provides an expression cassette for regulating fiber-selective expression in plants.

Fiber selective promoters

The present application discloses a chimeric gene comprising a promoter region consisting of the nucleotide sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 1437 operably linked to a nucleic acid coding for an expression product of interest, and a transcription termination and polyadenylation sequence. Also disclosed herein are a vector, a transgenic plant cell, a transgenic plant and a seed comprising such a chimeric gene. Methods disclosed herein relate to the production of a transgenic plant, growing cotton, producing a seed, effecting fiber-preferential expression of a product in cotton and of altering fiber properties in a cotton plant using the chimeric gene described.

Fiber-preferential promoter in cotton

Growing community of inventors

Merelbeke, Belgium

Frank Meulewaeter